Potenciador para Western Blot SuperSignal™
Potenciador para Western Blot SuperSignal™
Thermo Scientific™

Potenciador para Western Blot SuperSignal™

El potenciador para Western Blot SuperSignal contiene un reactivo de tratamiento de membrana y un diluyente de anticuerpos primarios queMás información
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Número de catálogoCantidad
46640500 mL
4664150 mL
Número de catálogo 46640
Precio (MXN)
-
Cantidad:
500 mL
Pedido a granel o personalizado
El potenciador para Western Blot SuperSignal contiene un reactivo de tratamiento de membrana y un diluyente de anticuerpos primarios que aumentan la intensidad y sensibilidad de la señal de 3 a 10 veces en comparación con una detección realizada sin él.

Características del potenciador para Western Blot SuperSignal:

• Aumente la sensibilidad: logre un aumento de 3 a 10 veces en la intensidad de la señal y sensibilidad
• Mejore la especificidad: mejora la relación señal-ruido para anticuerpos de mala calidad y baja afinidad
• Mayor claridad: reduce el fondo para conseguir unos Western Blots más limpios
• Compatibilidad de membrana: proporciona una mejora eficaz de la señal con membranas de PVDF y nitrocelulosa
• Compatibilidad de sustratos: se puede utilizar con métodos de detección cromogénicos, quimioluminiscentes y fluorescentes

Cuando una proteína o antígeno es difícil de detectar debido a una baja abundancia o mala inmunorreactividad, el uso de la tecnología de potenciador para Western Blot SuperSignal puede reducir significativamente el fondo, y mejorar la detección de antígenos inmunorreactivos de baja abundancia o baja inmunorreactividad.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónColorimétrico, quimioluminiscente, fluorescente
Para utilizar con (aplicación)Western Blot
FormularioLíquido
Membrane CompatibilityNitrocelulosa, PVDF
Línea de productosSuperSignal
Tipo de productoPotenciador para inmunotransferencia
Cantidad500 mL
Unit SizeEach
Contenido y almacenamiento
Tras la recepción, guardar las soluciones a 4 °C. Los productos se envían a temperatura ambiente.

Preguntas frecuentes

How can I reduce background with SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are potential causes for high background and corresponding solutions:
- Too much HRP in the system: Further dilute the HRP-conjugate, see guidelines in Table 1 in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018858_SuperSignalWestAtto_UG.pdf).
- Inadequate blocking: Optimize blocking conditions.
- Inappropriate blocking reagent: Try a different blocking reagent. Use clear milk or StartingBlock blocking buffer instead of dry milk.
- Inadequate washing: Increase length, number or volume of washes.
- Film has been overexposed: Decrease exposure time or use Pierce Background Eliminator (Cat No. 21065).
- Concentration of antigen or antibody is too high: Decrease amount of antigen or antibody.
- Poor antibody specificity: Use SuperSignal Western Blot Enhancer (Cat. No. 46640).
We also recommend minimizing handling of the membrane, using clean gloves while handling, clean plasticware, glassware, forceps, or other contact surfaces. We also recommend trimming of the membrane to remove excess area.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do I have to use a SuperSignal Western Blot substrate after using the SuperSignal Western Blot Enhancer?

Not necessarily. The SuperSignal Western Blot Enhancer will work with any chemiluminescent substrate (including SuperSignal Western Blot substrates), as well as colorimetric or fluorometric detection systems.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the difference between Western Blot Signal Enhancer and SuperSignal Western Blot Enhancer? Is there any benefit to one versus the other?

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

When should I use the SuperSignal Western Blot Enhancer (Cat. Nos. 46640 and 46641)?

The SuperSignal Western Blot Enhancer kit contains two reagents:

- Antigen Pretreatment Solution: used after transferring proteins to the membrane and before blocking or staining the membrane
- The Primary Antibody Diluent: used to dilute the primary antibody

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.