eBioscience™ Streptavidin eFluor™ 450 Conjugate

Los conjugados de estreptavidina y fluorocromo se utilizan habitualmente en protocolos de tinción indirecta para detectar anticuerpos primarios biotinilados enMás información

Número de catálogo	Cantidad
48-4317-82	100 μg

Número de catálogo 48-4317-82

Precio (MXN)

Cantidad:

100 μg

Los conjugados de estreptavidina y fluorocromo se utilizan habitualmente en protocolos de tinción indirecta para detectar anticuerpos primarios biotinilados en citometría de flujo. La estreptavidina se une a la biotina con alta afinidad.

Láser
Láser violeta

Emisión
445 nm

Excitación
405 nm

Aplicación declarada
Análisis mediante citometría de flujo

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Concentración0,2 mg/ml

Etiqueta o tinteeFluor 450

Tipo de productoConjugado de estreptavidina (Fluorescente)

Cantidad100 μg

Molécula dianaBiotina

ConjugadoeFluor 450

FormularioLíquido

Línea de productoseBioscience

Unit SizeEach

Preguntas frecuentes

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.