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Citas y referencias (11)
Gibco™
Medio de suero reducido Opti-MEM™ I, suplemento GlutaMAX™
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumMás información
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| Número de catálogo | Cantidad |
|---|---|
| 51985034 | 500 mL |
| 51985091 | 10 × 500 mL |
Número de catálogo 51985034
Precio (MXN)
-
Cantidad:
500 mL
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.
This Opti-MEM™ I is modified as follows:
| With |
| • GlutaMAX™ |
| • Phenol Red |
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.
Especificaciones
Línea de célulasSp2, AE-1, CHO, BHK-21 y HEK
Tipo de célulaFibroblastos primarios
Concentración1X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco, GlutaMAX, Opti-MEM
Tipo de productoOpti-MEM I
Cantidad500 mL
Duración de almacenamiento18 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónOrigen animal
FormularioLíquido
EsterilidadEstéril con filtro
Con aditivosGlutaMAX, Rojo de fenol
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 18 meses a partir de la fecha de fabricación
Condiciones de envío: Ambiente
Vida útil: 18 meses a partir de la fecha de fabricación
Preguntas frecuentes
Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?
Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?
Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?
I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?
How long can I keep my media after supplementing with serum?
Citations & References (11)
Citations & References
Abstract
Integrin-linked kinase regulates inducible nitric oxide synthase and cyclooxygenase-2 expression in an NF-kappa B-dependent manner.
Journal:J Biol Chem
PubMed ID:11724787
'Nitric oxide (NO) and prostaglandins are produced as a result of the stimulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2, respectively, in response to cytokines or lipopolysaccharide (LPS). We demonstrate that the activity of integrin-linked kinase (ILK) is stimulated by LPS activation in J774 macrophages. Inhibition of ILK activity
Doxorubicin induces apoptosis and CD95 gene expression in human primary endothelial cells through a p53-dependent mechanism.
Journal:J Biol Chem
PubMed ID:11779855
'Regulation of the homeostasis of vascular endothelium is critical for the processes of vascular remodeling and angiogenesis under physiological and pathological conditions. Here we show that doxorubicin (Dox), a drug used in antitumor therapy, triggered a marked accumulation of p53 and induced CD95 gene expression and apoptosis in proliferating human
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a
Phospholipase C-gamma is required for agonist-induced Ca2+ entry.
Journal:Cell
PubMed ID:12437926
'We report here that PLC-gamma isoforms are required for agonist-induced Ca2+ entry (ACE). Overexpressed wild-type PLC-gamma1 or a lipase-inactive mutant PLC-gamma1 each augmented ACE in PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of PLC-gamma1 was ineffective. RNA interference to deplete either PLC-gamma1 or PLC-gamma2
Hydrogen peroxide stimulates c-Src-mediated big mitogen-activated protein kinase 1 (BMK1) and the MEF2C signaling pathway in PC12 cells: potential role in cell survival following oxidative insults.
Journal:J Biol Chem
PubMed ID:11782488
'Reactive oxygen species, generated by reduction-oxidation (redox) reactions, have been recognized as one of the major mediators of ischemia and reperfusion injury in the brain. Reactive oxygen species-induced cerebral events are attributable, in part, to the change in intracellular signaling molecules including mitogen-activated protein (MAP) kinases. Big MAP kinase 1