Search
Thermo Scientific™
SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.
| Número de catálogo | Tamaño de partículas |
|---|---|
| 60160-110 | ˜20 μm |
Número de catálogo 60160-110
Precio (MXN)
-
Tamaño de partículas:
˜20 μm
Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.
SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.
The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.
Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.
Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Especificaciones
Formato de columnaImmobilized enzyme
Tipo de columnaImmobilized enzyme in PEEK cartridge
DescripciónSMART Digest Pepsin/Protease XIII Dual Enzyme Column
Diámetro2.1 mm
Caudal10 to 500 μL/min
Para utilizar con (aplicación)HDX-MS analysis
Longitud30 mm
Presión máxima2500 psi (170 bar)
Compatibilidad de fase móvilPhosphate buffer pH 4
Intervalo de temperatura de funcionamiento4 °C to 50 °C
Material de embalajeHydrophilic/coated DVB
Tamaño de partículas˜20 μm
Tamaño de poro° 1000 angstroms
Estabilidad1 year
Fase estacionariaImmobilized enzyme
Temperatura de almacenamientoRT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
TécnicaBioLC
Peso8 g
CleavagePepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
EnzimaPepsin & Protease XIII
TipoSMART Digest Pepsin/Protease XIII Dual Enzyme column
pH2 to 6
Unit SizeEach