Slide-A-Lyzer™ Dialysis Flotation Disks, 6 Disks

It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Most likely it‘s because osmotic pressure from dialyzing from high osmolarity to low osmolarity solutions causes a sample volume increase, as each sample component-including water-will move towards equilibrium concentration on both sides of the membrane.

Typical protein recovery is greater than 90% after dialysis.

The plate comprises detachable strips so that you can pull out the exact number of wells needed for an experiment (scalable from 1-96 samples).

The Slide-A-Lyzer Dialysis Cassette is the same regenerated cellulose as other dialysis tubing so you can expect about the same amount of protein loss as with tubing. As a guide, a 1 mg/mL solution will have a recovery rate of greater than 95%; at 100 mg/mL the recovery drops to 75-80%; and in solutions as dilute 10 µg/mL users may observe only a 50% (or less) recovery.