eBioscience™ BrdU Staining Kit for Flow Cytometry FITC

El kit de tinción BrdU de eBioscience para citometría de flujo contiene los reactivos y tampones necesarios para identificar yMás información

Número de catálogo	Cantidad
8811-6600-42	100 pruebas

Número de catálogo 8811-6600-42

Precio (MXN)

Cantidad:

100 pruebas

El kit de tinción BrdU de eBioscience para citometría de flujo contiene los reactivos y tampones necesarios para identificar y examinar células proliferantes de especies de mamíferos mediante el análisis de citometría de flujo. Las células en ciclo se incuban con 5-bromo-2’-desoxiuridina (BrdU), un análogo sintético de la timidina que se incorpora al ADN genómico recién sintetizado durante la fase S de la mitosis. Tras la desnaturalización del ADN, las células se tiñen para incorporarlas al BrdU junto con cualquier otra superficie celular u objetivo intracelular de interés.

Este kit está optimizado para lograr una tinción más brillante en comparación con los protocolos tradicionales.

Reactividad/especie
Bobina, canina, vaca, humano, mono, ratón, primate no humano, rata, mono Resus

Conjugado
Láser

Láser azul
FITC

Emisión
520 nm

Excitación
488 nm

Aplicación declarada
Tinción intracelular seguida de análisis de citometría de flujo

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

FormatoKit

Cantidad100 pruebas

Para utilizar con (aplicación)Tinción de BrdU

Para utilizar con (equipo)Citómetro de flujo

Línea de productoseBioscience

Tipo de productosecciones congeladas Shandon

Unit SizeEach

Preguntas frecuentes

Can I also stain for intracellular targets such as transcription factors and cytokines using the eBioscience BrdU staining kit?

Yes, primary antibodies against additional intracellular targets can be added alongside the Anti-BrdU antibody. However, it is critical that you empirically determine whether these additional antibodies are compatible with this staining protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the BrdU in the eBioscience BrdU staining kit supplied as a sterile formulation for in vivo use?

Yes, the BrdU is supplied as a sterile liquid. The vial should be opened under sterile conditions if it will be used in vivo.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I keep the diluted (1 mM) BrdU in the eBioscience BrdU staining kit at 4 degrees C? Can I refreeze it after use?

The stock (32.5 mM) BrdU can be frozen and thawed up to three times. Furthermore, the stock BrdU can be kept at 4 degrees C for up to one week. However, the diluted (1 mM) BrdU should be used immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Have the BrdU Staining kits been tested on proliferating cells of different species?

We have used this kit to detect proliferating human and mouse cells. Other species have yet to be tested but feline, non-human primate and canine should also work.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the stopping points during flow cytometric analysis when using a BrdU staining kit?

Samples can be stored at 4 degrees C for up to 16 hours (i.e., overnight) after fixation with BrdU Staining Buffer (with or without washing in Flow Cytometry Staining Buffer) with no adverse effect on performance.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all eBioscience™ BrdU Staining Kit for Flow Cytometry FITC FAQs