StemPro™ MSC SFM

Citas y referencias (13)

Gibco™

StemPro™ MSC SFM

StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs).

Número de catálogo	Con aditivos	Sin aditivos
A1033201	Bajo nivel de glucosa, Piruvato sódico, Bicarbonato sódico, Adenosine, Desoxirribonucleósidos	Sin glutamina, Sin rojo fenol

Número de catálogo A1033201

Precio (MXN)

Con aditivos:

Bajo nivel de glucosa, Piruvato sódico, Bicarbonato sódico, Adenosine, Desoxirribonucleósidos

Sin aditivos:

Sin glutamina, Sin rojo fenol

StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs). StemPro™ MSC SFM enables superior human MSC growth and increased consistency compared to classical serum-supplemented medium (DMEM + 10% FBS).

Using StemPro™ MSC SFM, human MSCs can be expanded beyond 5 passages while still maintaining their tri-lineage mesoderm differentiation potential (i.e., ability to differentiate into osteogenic, chondrogenic and adipogenic lineages). Each lot of StemPro™ MSC SFM is qualified using a human MSC performance assay and human MSCs growing in serum-supplemented medium can be transitioned directly into StemPro™ MSC SFM with little or no adaptation required. In addition, StemPro™ MSC SFM facilitates expansion of MSCs directly from primary human bone marrow.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas madre mesenquimales

Entorno de cultivoCO₂

Nivel de endotoxinas≤10 EU/ml

Sales inorgánicasCalcio, fosfato sódico

Línea de productosStemPro

Tipo de productoMedio libre de suero (SFM) de células madre

Grado de pureza o calidadCalidad para investigación

Cantidad1 Kit

EspecieHumano

ClasificaciónSin suero

Culture TypeCultivo celular adherente

FormularioLíquido

Serum LevelSin suero

Con aditivosBajo nivel de glucosa, Piruvato sódico, Bicarbonato sódico, Adenosine, Desoxirribonucleósidos

Sin aditivosSin glutamina, Sin rojo fenol

Unit SizeEach

Contenido y almacenamiento

Este kit se envía en dos piezas con requisitos de almacenamiento independientes. Estos componentes no se venden por separado:
• 500 ml de medio basal MSC SFM StemPro™ (almacenar en un lugar oscuro a una temperatura de entre 2 °C y 8 °C)
• 75 ml de suplemento MSC SFM StemPro™ (almacenar en un lugar oscuro a una temperatura de entre – 5 °C y – 20 °C)

El medio basal se envía a temperatura ambiente, mientras que el suplemento se envía en hielo seco.

Preguntas frecuentes

Can StemPro MSC SFM (Cat. No. A1003201) be used to isolate primary cells?

Yes. StemPro MSC SFM (Cat. No. A003201) can be used for both isolating primary cells and transitioning cells from serum-containing medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do StemPro MSC SFM Basal Medium CTS and StemPro MSC SFM Supplement CTS contain L-Glutamine?

Both CTS StemPro MSC SFM and StemPro MSC SFM Supplement (Cat. No. A1033201) do not contain L-Glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

View all StemPro™ MSC SFM FAQs

Citations & References (13)

Citations & References

Abstract

PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages.

Authors:Ng F, Boucher S, Koh S, Sastry KS, Chase L, Lakshmipathy U, Choong C, Yang Z, Vemuri MC, Rao MS, Tanavde V,

Journal:Blood

PubMed ID:18332228

We compared the transcriptomes of marrow-derived mesenchymal stem cells (MSCs) with differentiated adipocytes, osteocytes, and chondrocytes derived from these MSCs. Using global gene-expression profiling arrays to detect RNA transcripts, we have identified markers that are specific for MSCs and their differentiated progeny. Further, we have also identified pathways that MSCs ... More

Ischemic Preconditioning Potentiates the Protective Effect of Stem Cells through Secretion of Exosomes by Targeting Mecp2 via miR-22.

Authors:Feng Y, Huang W, Wani M, Yu X, Ashraf M,

Journal:

PubMed ID:24558412

Mesenchymal stem cells (MSCs) have potential application for the treatment of ischemic heart diseases. Besides differentiation properties, MSCs protect ischemic cardiomyocytes by secretion of paracrine factors. In this study, we found exosomes enriched with miR-22 were secreted by MSCs following ischemic preconditioning (Exo(IPC)) and mobilized to cardiomyocytes where they reduced ... More

Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy.

Authors:Riis S, Zachar V, Boucher S, Vemuri MC, Pennisi CP, Fink T,

Journal:

PubMed ID:26052798

Since the discovery of adipose-derived stem cells (ASCs), there have been high expectations of their putative clinical use. Recent advances support these expectations, and it is expected that the transition from pre-clinical and clinical studies to implementation as a standard treatment modality is imminent. However ASCs must be isolated and ... More

Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro.

Authors:Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, Haataja R, Vemuri M, Suuronen R, Miettinen S,

Journal:Cytotherapy

PubMed ID:19903107

BACKGROUND AIMS: Human adipose stem cells (ASC) are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue. ASC have been shown to have therapeutic applicability in pre-clinical studies, but a standardized expansion method for clinical cell therapy has yet to be established. Isolated ASC are ... More

Growth, differentiation capacity, and function of mesenchymal stem cells expanded in serum-free medium developed via combinatorial screening.

Authors:Crapnell K, Blaesius R, Hastings A, Lennon DP, Caplan AI, Bruder SP

Journal:Exp Cell Res

PubMed ID:23597555

The presence of serum in cell culture medium presents an obstacle to safe and efficient production of hMSCs for therapeutic purposes. Availability of defined medium will be crucial to elucidating the mechanism of action of hMSCs in many indications as well as a prerequisite to consistently produce cells with predictable ... More

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