AbC™ Anti-Mouse Bead Kit

Citas y referencias (3)

Invitrogen™

AbC™ Anti-Mouse Bead Kit

El kit de gránulos de compensación de anticuerpos antimurinos AbC™ proporciona una técnica uniforme, precisa y sencilla para el ajusteMás información

Número de catálogo	Cantidad
A10344	1 kit

Número de catálogo A10344

Precio (MXN)

Cantidad:

1 kit

El kit de gránulos de compensación de anticuerpos antimurinos AbC™ proporciona una técnica uniforme, precisa y sencilla para el ajuste de la compensación de citometría de flujo cuando se utilizan anticuerpos de ratón conjugados con fluorocromo con cualquier láser en citometría de flujo.

• Reactividad de las especies: para su uso con todos los isotipos de anticuerpos de ratón conjugados
• Máxima compatibilidad con láseres: para su uso con todo tipo de láseres

La compensación precisa es crucial para un análisis eficaz de múltiples colores en citometría de flujo. A menudo, sin embargo, los controles de compensación con base celular no son completamente eficaces, ya que muchos antígenos no presentan una elevada expresión y las células débilmente teñidas pueden llevar a ajustes de compensación inexactos. El kit de gránulos antirratón AbC™ se ha desarrollado para solucionar este problema.

Consulte una guía de selección para todos los gránulos de compensación para citometría de flujo

Cómo funciona
Cada kit proporciona microesferas de poliestireno que tienen capacidad para capturar conjugados de anticuerpos fluorescentes (gránulos de compensación positiva) o inertes (gránulos de compensación negativa). Se mezclan con un anticuerpo de ratón conjugado con un fluoróforo, los dos componentes proporcionan un control positivo de alta señal distinto con una población negativa adecuada que se puede utilizar para establecer la correcta compensación, independientemente de la intensidad de las células en el experimento real.

Reactividad de las especies
El kit de gránulos antirratón AbC™ es ideal para la compensación con todos los isotipos de inmunoglobulinas de ratón (IgG1, IgG2a, IgG2b, IgG3, IgM).

Máxima compatibilidad con láseres
Todos los kits de gránulos de compensación AbC™ son ideales para su uso con cualquier fuente de excitación, incluido el láser de 405 nm que históricamente ha proporcionado señales de fondo más altas debido a la autofluorescencia con otros kits comerciales.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Diámetro (métrico)6 μm

Intervalo de longitud de onda de excitacióncualquier láser (UV a 633/635)

Para utilizar con (equipo)Citómetro de flujo

FormatoTubo

Nº de pruebas100 tests

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

Configuración de tinciónSuperficie

Línea de productosCompenFlow, Molecular Probes

Tipo de productoKit de gránulos de compensación de anticuerpos

Unit SizeEach

Contenido y almacenamiento

Contiene 1 vial de gránulos de captura AbC™ y 1 vial de gránulos negativos.
Almacenar a 2-8 °C.

Preguntas frecuentes

I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?

It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I’ve been told that I need to compensate my data for flow cytometry analysis. What does that mean?

By running single-color controls, it is possible to remove signal of one fluorophore that spills over into the collection channel for another fluorophore.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kind of controls do I need for flow cytometry?

For compensation, you need to prepare a singly stained sample (or compensation beads) for each color parameter that you are using. In addition, we recommend that you use FMO (flow minus one) controls. These are controls in which you label cells or beads with every color in your panel, omitting one. Make one FMO control for each color. These controls are important for helping you properly set gates on your data.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why should I worry about compensation in flow cytometry analysis?

In a perfect world, the fluorescence emission profile for each individual fluorophore would be a very intense, narrow peak, well separated from all other emission peaks. In reality, organic dyes and fluorescent proteins have broad emission peaks, and compensation must be employed (during or after data acquisition) to correctly assign fluorescence signal to each fluorophore. Compensation is important because it removes fluorescent signal from overlapping spectra so you know that the signal you see is only the signal from the fluorophore of interest.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I make compensation controls for antibodies?

All that you need for compensation controls is an unstained sample of your cells (or negative control beads) and samples with each of your fluorophores - one per tube. You want the single-stained controls to be as bright as you expect your brightest sample to be. Antibodies can be bound to beads instead of cells using our AbC Total Antibody Compensation Bead Kit (Cat. Nos. A10513 and A10497).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (3)

Citations & References

Abstract

The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation.

Authors:Barbera M, di Pietro M, Walker E, Brierley C, Macrae S, Simons BD, Jones PH, Stingl J, Fitzgerald RC,

Journal:

PubMed ID:24572143

Knowledge of the cellular mechanisms involved in homeostasis of human squamous oesophagus in the steady state and following chronic injury is limited. We aimed to better understand these mechanisms by using a functional 3D approach. Proliferation, mitosis and the expression of progenitor lineage markers were assessed in normal squamous oesophagus ... More

Haemocompatibility and ion exchange capability of nanocellulose polypyrrole membranes intended for blood purification.

Authors:Ferraz N, Carlsson DO, Hong J, Larsson R, Fellström B, Nyholm L, Strømme M, Mihranyan A,

Journal:J R Soc Interface

PubMed ID:22298813

Composites of nanocellulose and the conductive polymer polypyrrole (PPy) are presented as candidates for a new generation of haemodialysis membranes. The composites may combine active ion exchange with passive ultrafiltration, and the large surface area (about 80 m(2) g(-1)) could potentially provide compact dialysers. Herein, the haemocompatibility of the novel ... More

Immunophenotype and cytokine profiles of rhesus monkey CD56bright and CD56dim decidual natural killer cells.

Authors:Dambaeva SV, Durning M, Rozner AE, Golos TG

Journal:Biol Reprod

PubMed ID:21900681

The primate endometrium is characterized in pregnancy by a tissue-specific population of CD56(bright) natural killer (NK) cells. These cells are observed in human, rhesus, and other nonhuman primate decidua. However, other subsets of NK cells are present in the decidua and may play distinct roles in pregnancy. The purpose of ... More