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Citas y referencias (18)
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ARP (N-(aminooxyacetyl)-N'-(D-Biotinoyl) hydrazine, Trifluoroacetic Acid Salt)
ARP (Aldehyde-Reactive Probe) es una hidroxilamina biotinilada que reacciona con el grupo de aldehído expuesto formado en sitios abásicos enMás información
| Número de catálogo | Cantidad |
|---|---|
| A10550 | 10 mg |
Número de catálogo A10550
Precio (MXN)
-
Cantidad:
10 mg
ARP (Aldehyde-Reactive Probe) es una hidroxilamina biotinilada que reacciona con el grupo de aldehído expuesto formado en sitios abásicos en ADN dañado. Las biotinas ligadas a los sitios abásicos se pueden cuantificar con complejos fluorescentes o de estreptavidina conjugados con enzimas. ARP es adecuado para su uso en ensayos de microplacas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaÁcido carboxílico, cetona, aldehído
Etiqueta o tinteBiotina
Tipo de productoARP
Cantidad10 mg
Fracción reactivaAmina, hidroxilamina
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaBiotina y otros haptenos
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).
Citations & References (18)
Citations & References
Abstract
Optimizing antibody immobilization strategies for the construction of protein microarrays.
Journal:Anal Biochem
PubMed ID:12531195
'Antibody microarrays have the potential to revolutionize protein expression profiling. The intensity of specific signal produced on a feature of such an array is related to the amount of analyte that is captured from the biological mixture by the immobilized antibody (the "capture agent"). This in turn is a function
N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts.
Journal:J Biol Chem
PubMed ID:10702229
'Alpha-phenyl-N-t-butyl nitrone (PBN), a spin trap, scavenges hydroxyl radicals, protects tissues from oxidative injury, and delays senescence of both normal human lung fibroblasts (IMR90) and senescence-accelerated mice. N-t-butyl hydroxylamine and benzaldehyde are the breakdown products of PBN. N-t-Butyl hydroxylamine delays senescence of IMR90 cells at concentrations as low as 10
Detection of abasic sites and oxidative DNA base damage using an ELISA-like assay.
Journal:Methods
PubMed ID:11020331
'Reactive oxygen species produce a wide spectrum of DNA damage, including oxidative base damage and abasic (AP) sites. Many procedures are available for the quantification and detection of base damage and AP sites. However, either these procedures are laborious or the starting materials are difficult to obtain. A biotinylated aldehyde-specific
Direct visualization of abasic sites on a single DNA molecule using fluorescence microscopy.
Journal:Photochem Photobiol
PubMed ID:12194206
'A new method was developed to allow direct visualization of damaged sites on individual DNA molecules. Fluorescence in situ hybridization on extended DNA molecules was modified to detect a single abasic site. Abasic sites were specifically labeled with a biotinylated aldehyde-reactive probe and fluorochrome-conjugated streptavidin. The light emitted by a
A chemoenzymatic approach toward the rapid and sensitive detection of O-GlcNAc posttranslational modifications.
Journal:J Am Chem Soc
PubMed ID:14692737