Human Mammary Epithelial Cells (HMEC)
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Human Mammary Epithelial Cells (HMEC)

Células primarias epiteliales mamarias humanas, criopreservadas al final del sexto cultivo.• Viabilidad de al menos el 70 %• ≥ 500Más información
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Número de catálogoCantidad
A105651 vial
Número de catálogo A10565
Precio (MXN)
-
Cantidad:
1 vial
Células primarias epiteliales mamarias humanas, criopreservadas al final del sexto cultivo.

• Viabilidad de al menos el 70 %
• ≥ 500 000 células viables / vial
• Capaz de al menos 16 duplicaciones de población
• Micoplasma: No se detecta
• Hepatitis B: No se detecta
• Hepatitis C: No se detecta
• VIH-1: No se detecta
• Bacterias, levaduras y otros hongos: No se detecta
• Positivo en citoqueratinas 5⁄6, 8, 18 y e-cadherina
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Fuente de dadoresDonante único
N.° de celdas≥500000
Línea de productosCascade Biologics
Tipo de productoCélula epitelial mamaria humana
Cantidad1 vial
Condiciones de envíoHielo seco
Probado paraHepatitis B (VHB), Hepatitis C (VHC), pruebas de esterilidad (hongos y bacterias), micoplasma, ensayo de rendimiento del crecimiento, VIH-1
AgeAdulta
Tipo de célulaCélulas epiteliales (mamarias)
Culture TypeCultivo celular adherente
FormularioCrioconservado
EspecieHumano
Unit SizeEach
Contenido y almacenamiento

Temperatura del nitrógeno líquido

Preguntas frecuentes

Is it difficult to culture/passage Human Mammary Epithelial Cells (HMEC)?

No. HMEC cultures are easily initiated at 2.5 x 10E3 cells per cm2 in standard tissue culture plasticware and passaged using a trypsin/EDTA solution.

How many T-25 flasks can I seed from 1 vial of Human Mammary Epithelial Cells (HMEC)?

One vial (500,000 viable cells) can seed eight T-25 flasks at 2,500 viable cells per cm2. A hemocytometer should be used to accurately count cells and to help ensure an appropriate seeding density.

What is the difference between M171 and HuMEC growth media?

Both media are designed for culture of HMEC. However, in-house studies show HuMEC Ready Medium (Thermo Fisher Scientific Cat. No. 12752-010) outperforms M171/MEGS (Thermo Fisher Scientific Cat. No. M-171-500/S-015-5) in both growth rate and lifespan measurements of HMEC. There is no difference between media when staining for cell-specific markers using indirect ICC.

How does passage number correlate with population doublings for Human Mammary Epithelial Cells (HMEC)?

One passage is equivalent to approximately 4 population doublings when using the recommended subculture conditions.

What are typical doubling times for Human Mammary Epithelial Cells (HMEC)?

HMEC usually have population doubling times of 24-30 hr during the guaranteed lifespan.

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