CHO DG44 Cells (cGMP banked) and Media Kit
CHO DG44 Cells (cGMP banked) and Media Kit
Gibco™

CHO DG44 Cells (cGMP banked) and Media Kit

El kit de medios y células CHO DG44 (procedentes de bancos según las cGMP) es un medio definido químicamente optimizadoMás información
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Número de catálogoCantidad
A11000011 kit
Número de catálogo A1100001
Precio (MXN)
-
Cantidad:
1 kit
El kit de medios y células CHO DG44 (procedentes de bancos según las cGMP) es un medio definido químicamente optimizado para el crecimiento de células de ovario de hámster chino (CHO) deficiente de dihidrofolato reductasa (DHFR-) y la expresión de proteínas recombinantes en cultivo de suspensión.El kit proporciona:

Medio CD DG44 GIBCO™: medio sin proteínas definido químicamente, específicamente diseñado para permitir el crecimiento y la expresión óptimos de las células DG44 en el cultivo en suspensión

Células CHO DG44 (procedentes de bancos según las cGMP): las células parentales CHO DG44 se han producido, almacenado y probado para cumplir con los estándares de calidad cGMP.Están preadaptados al medio CD DG44 y seleccionados para un crecimiento celular superior y eficiencias de transfección.

Además, las células CHO DG44 (procedentes de bancos según las cGMP) y el kit de medios incluyen L-glutamina y Pluronic™ F-68 para un crecimiento óptimo y la expresión de proteínas de las células DG44 en un entorno sin proteínas y definido químicamente.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ClasificaciónSin material de origen animal, definido químicamente, sin proteínas, sin suero
Recomendación de mediosMedio CD/DG44
N.° de celdas1 x 107
Línea de productosGibco
Cantidad1 kit
Línea de célulasCHO DG44
Culture TypeCultivos celulares en suspensión
EspecieHámster
Unit SizeEach
Contenido y almacenamiento
Las células CHO DG44 (cGMP en bancos) y el kit de medios (SKU A1100001) contienen:

• Medio CD DG44 de 1000 ml - 12610-010, almacenar en la oscuridad de 2 a 8 °C
• Células CHO DG44 (procedentes de bancos según las cGMP) 1 vial (>1 x 10^7 células) - A1097101 (ver licencia de etiqueta limitada), almacenar en nitrógeno líquido
• PLURONIC F-68, 10 % (100X) 100 ml - 24040-032, almacenar a temperatura ambiente
• L-glutamina-200mM (100X) 100 ml - 25030-081 o 25030-024 (versión con marcado CE solo para Europa), almacenar a -20 °C

Preguntas frecuentes

Has the DG44 cell line been used for the large scale production of any FDA-approved biotherapeutic proteins?

70% of all therapeutics produced in mammalian cells are expressed in CHO cells. CHO cells are preferred because of human-like glycosylation and no propagation of human-like viruses. Most therapeutic IgGs are produced using the CHO platform and a number of FDA-approved biotherapeutics have been produced in DG44 cells.

Why are the defrosted CHO DG44 Cells (cGMP banked) transferred directly into pre-warmed media instead of being centrifuged for recovery when using the CHO DG44 Cells (cGMP banked) and Media Kit (Cat. No. A1100001)?

The defrosted contents of the vial of Gibco CHO DG44 Cells are transferred directly to 29 mL of pre-warmed media and then incubated because the cells are extremely fragile upon recovery from cryopreservation. Centrifuging the cells at this stage could cause significant damage, reducing their viability and recovery efficiency. To help ensure optimal recovery and viability, the cells are directly transferred to pre-warmed media and incubated under optimal conditions. This approach is also why we do not mention centrifuge resuspension in the subculture section. We have found that the shear forces during centrifugation are more harmful to the cells than retaining a small amount of old media or DMSO. The volume of media in the flask has been found to sufficiently dilute the DMSO such that it does not impact recovery or cell growth rather than using a centrifuge.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I learn more about commercial licensing of Freedom kits, ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

To inquire about a Commercial Production or Service License, please email: outlicensing@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the term of the license agreement for ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

Licensing rights are granted in perpetuity for the Commercial Production License. The term of the Commercial Service License is negotiated between the parties.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is there a pull-through royalty on final products or an annual maintenance fee for using ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)??

Only a one-time license fee is required for a Commercial Production License, and it covers use during the clinical trial process and commercialization of an unlimited number of molecules.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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