Citas y referencias (85)

Invitrogen™

Phalloidin Labeling Probes

Name: Phalloidin Labeling Probes
SKU: A12380

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.

Cambiar vista

Número de catálogo	Color	Intervalo de longitud de onda de excitación	Tipo de colorante
A12380	Rojo anaranjado	578⁄600	Alexa Fluor™ 568
A22281	Azul	346⁄442	Alexa Fluor™ 350
A30104	Violeta	405/450	Alexa Fluor™ Plus 405
A12379	Verde	495⁄518	Alexa Fluor™ 488
O7466	Verde	496⁄520	Oregon Green™ 488
F432	Verde	496⁄516	FITC (fluoresceína)
A22282	Amarillo	531⁄554	Alexa Fluor™ 532
R415	Rojo anaranjado	540⁄565	TRITC (isotiocianato de tetrametilrodamina)
A22283	Naranja	556⁄570	Alexa Fluor™ 546
A34055	Naranja	555⁄565	Alexa Fluor™ 555
A30106	Naranja	555/565 nm	Alexa Fluor Plus 555
B3475	Rojo	558⁄569	BODIPY™
A12381	Rojo	581⁄609	Alexa Fluor™ 594
T7471	Rojo	591⁄608	Texas Red™
A22284	Rojo lejano	632⁄647	Alexa Fluor™ 633
A34054	Rojo lejano	633⁄647	Alexa Fluor™ 635
A22287	Rojo lejano	650⁄668	Alexa Fluor™ 647
A30107	Rojo lejano	650/668 nm	Alexa Fluor Plus 647
A22285	Infrarrojo cercano	663⁄690	Alexa Fluor™ 660
A22286	Infrarrojo cercano	679⁄702	Alexa Fluor™ 680
A30105	Infrarrojo cercano	758/784	Alexa Fluor™ Plus 750
B7474	Ninguno	Ninguno	Biotin-XX
P3457	Ninguno	Ninguno	Faloidina (sin marcar)

23 opciones

Número de catálogo A12380

Precio (MXN)

Color:

Rojo anaranjado

Intervalo de longitud de onda de excitación:

578⁄600

Tipo de colorante:

Alexa Fluor™ 568

Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
Ease of use—staining is straightforward and quick
Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo anaranjado

Tipo de coloranteAlexa Fluor™ 568

Intervalo de longitud de onda de excitación578⁄600

Para utilizar con (equipo)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with Texas Red filter set

Línea de productosAlexa Fluor

Cantidad300 Units

Condiciones de envíoTemperatura ambiente, Temperatura ambiente

Tipo de etiquetaColorantes Alexa Fluor

Tipo de productoFaloidina

Sub Cellular LocalizationActina, citoesqueleto, Cytoskeleton

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

Do you offer alternative package sizes for phalloidin or ReadyProbes reagents?

Alternative package sizes may be available through a custom request process. Contact Thermo Fisher Scientific customer support or your sales representative to discuss available options for the specific reagent and quantity needed.

Can I use conjugated phalloidin label probes on organoids?

Yes. Conjugated phalloidin labeling probes can be used with organoids when F-actin is accessible after fixation and permeabilization. Because organoids are thicker than monolayer cell cultures, protocol optimization may be needed, including permeabilization conditions, probe incubation time, and probe concentration.

Are conjugated phalloidin reagents antibodies?

No, Phalloidin is not an antibody. It is a bicyclic peptide toxin originally isolated from the Amanita phalloides mushroom and is used to bind and label F-actin in fixed and permeabilized samples.

Can I use methanol instead of DMSO to reconstitute the conjugated phalloidins from the lyophilized solids?

Yes, methanol can be used to reconstitute fluorescent phalloidin, but it creates a less concentrated stock solution than DMSO. Dissolve the vial contents in 1.5 mL of methanol to yield a 40X stock solution at a concentration of 200 assays/mL, which is equivalent to approximately 6.6 µM. One unit/assay of fluorescent phalloidins is equivalent to 5 µL of the methanolic stock solution. Methanol may be used as a stock-solution solvent, but methanol-containing fixatives are not recommended because they can disrupt F-actin.

How do I reconstitute fluorescent conjugated phalloidin probes to make a stock solution?

For fluorescent phalloidin label probes, prepare a DMSO stock solution by dissolving the vial contents in 150 µL anhydrous DMSO to make a 400X stock, with a concentration of approximately 66 µM or 2,000 assays per mL. Store between -25°C and -5°C, protected from light. One unit/assay of fluorescent phalloidins is equivalent to 0.5 µL of the DMSO stock solution.

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Citations & References (85)

Citations & References

Abstract

Control of integrin alphaIIb beta3 outside-in signaling and platelet adhesion by sensing the physical properties of fibrin(ogen) substrates.

Authors:Podolnikova NP, Yermolenko IS, Fuhrmann A, Lishko VK, Magonov S, Bowen B, Enderlein J, Podolnikov AV, Ros R, Ugarova TP,

Journal:Biochemistry

PubMed ID:19929007

The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of ... More

Phosphocaveolin-1 is a mechanotransducer that induces caveola biogenesis via Egr1 transcriptional regulation.

Authors:Joshi B, Bastiani M, Strugnell SS, Boscher C, Parton RG, Nabi IR,

Journal:J Cell Biol

PubMed ID:23091071

'Caveolin-1 (Cav1) is an essential component of caveolae whose Src kinase-dependent phosphorylation on tyrosine 14 (Y14) is associated with regulation of focal adhesion dynamics. However, the relationship between these disparate functions remains to be elucidated. Caveola biogenesis requires expression of both Cav1 and cavin-1, but Cav1Y14 phosphorylation is dispensable. In ... More

Micron-scale spatially patterned, covalently immobilized vascular endothelial growth factor on hydrogels accelerates endothelial tubulogenesis and increases cellular angiogenic responses.

Authors:Leslie-Barbick JE, Shen C, Chen C, West JL,

Journal:Tissue Eng Part A

PubMed ID:20712418

'Spontaneous formation of endothelial tubules was restricted to patterned micron-scale regions presenting cell adhesion ligands and angiogenic signaling protein on poly(ethylene glycol) hydrogels. Arginine-glycine-aspartic acid-serine (RGDS), an integrin ligand, and vascular endothelial growth factor (VEGF), a rate-limiting signaling protein involved in angiogenesis, were covalently bound through photopolymerization via laser scanning ... More

Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5).

Authors:Futter M, Uematsu K, Bullock SA, Kim Y, Hemmings HC, Nishi A, Greengard P, Nairn AC

Journal:Proc Natl Acad Sci U S A

PubMed ID:15728359

'Spinophilin is a protein that binds to protein phosphatase-1 and actin and modulates excitatory synaptic transmission and dendritic spine morphology. We have identified three sites phosphorylated by ERK2 (Ser-15 and Ser-205) and cyclin-dependent PK 5 (Cdk5) (Ser-17), within the actin-binding domain of spinophilin. Cdk5 and ERK2 both phosphorylated spinophilin in ... More

A high-throughput, cell-based screening method for siRNA and small molecule inhibitors of mTORC1 signaling using the In Cell Western technique.

Authors:Hoffman GR, Moerke NJ, Hsia M, Shamu CE, Blenis J,

Journal:Assay Drug Dev Technol

PubMed ID:20085456

'The mTORC1 pathway is a central regulator of cell growth, and defective mTORC1 regulation plays a causative role in a variety of human diseases, including cancer, tumor syndromes such as the tuberous sclerosis complex (TSC) and lymphangioleiomyomatosis (LAM), and metabolic diseases such as diabetes and obesity. Given the importance of ... More

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