Conjugados de anexina V para la detección de apoptosis
Conjugados de anexina V para la detección de apoptosis
Invitrogen™

Conjugados de anexina V para la detección de apoptosis

Detecte las primeras etapas de la apoptosis con la anexina V independiente Alexa Fluor, APC, Pacific Blue, PE, FITC y conjugados de biotina mediante citometría de flujo.
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Número de catálogoExcitación/emisiónLíneas láser del citómetro de flujoConjugado
A13204Biotina X
A13201495/519488Alexa Fluor 488
A13199494/518488FITC
A13202578/603532, 561Alexa Fluor 568
A13203590/617532Alexa Fluor 594
A23202346/442UVAlexa Fluor 350
A23204650/665633-637Alexa Fluor 647
A35108555/565532, 561Alexa Fluor 555
A35109679/702633-637Alexa Fluor 680
A35110650/660633-637APC (Aloficocianina)
A35111565/578488, 532, 561PE
A35122410/455405Pacific Blue
Número de catálogo A13204
Precio (MXN)
-
Conjugado:
Biotina X
Consiga una detección rápida y fiable de la apoptosis celular temprana con conjugados independientes de anexina V para la detección de apoptosis. Los conjugados de anexina V ofrecen una diferencia de hasta 100 veces en la intensidad de la señal de fluorescencia entre células apoptóticas y no apoptóticas mediante citometría de flujo.
La anexina V tiene una alta afinidad a la fosfatidilserina (PS), que se expone en el recubrimiento externo de las células que experimentan apoptosis. Debido a esta afinidad, los reactivos de anexina V etiquetados fluorescentemente se utilizan comúnmente en la investigación de la apoptosis.

Los conjugados de anexina V proporcionan métodos de detección rápidos y fiables para estudiar la externalización de la fosfatidilserina, un indicador de las etapas intermedias de la apoptosis. La diferencia en la intensidad de la fluorescencia entre las células apoptóticas y no apoptóticas teñidas con nuestros conjugados de anexina V fluorescente, medida por citometría de flujo, suele ser de aproximadamente 100 veces.

En colaboración con Nexins Research BV, proporcionamos los mejores y más brillantes conjugados de anexina V disponibles, incluidos los conjugados de anexina V Alexa Fluor 350, 488, 555, 568, 594, 647 y 680, así como los conjugados de anexina V APC, biotina-X, FITC, Pacific Blue y PE. Los conjugados de anexina V altamente fluorescente proporcionan métodos de detección rápidos y fiables para el estudio de la externalización de la fosfatidilserina, uno de los primeros indicadores de apoptosis.

El conjugado de anexina V Pacific Blue es excitable con violeta, lo que lo hace ideal para instrumentos con un láser violeta y para experimentos multicolor que incluyan colorantes verdes o rojos fluorescentes.

Los beneficios de nuestros conjugados de anexina V incluyen:
• Conjugado con los colorantes Invitrogen Alexa Fluor y eFluor para señales más brillantes
• Conjugado para todos los láseres disponibles
• Disponible como reactivos independientes o kits fáciles de usar

La tinción de anexina V para detectar células apoptóticas solo se puede realizar en células y tejidos vivos. Si las muestras se van a fijar después de la tinción, se requieren condiciones específicas para lograr la retención transitoria de la señal. Estas incluyen el uso de un método de fijación a base de aldehídos sin alcohol, el uso de tampones que contengan Ca2+ y evitar los tensioactivos/detergentes. Para su comodidad, también ofrecemos un tampón concentrado de unión a anexina que facilita la unión de la anexina V a la fosfatidilserina en ensayos de apoptosis.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
DescripciónAnexina V, conjugado de biotina-X.
Para utilizar con (equipo)Citómetro de flujo
Contenido del kitContiene un vial de anexina V, conjugado de biotina-x.
N.º de reacciones100
Tipo de productoConjugado de anexina V
Cantidad500 μL
Condiciones de envíoHielo húmedo
ConjugadoBiotina X
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador a una temperatura de entre 2 y –8°C.

Preguntas frecuentes

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am trying to label adherent cells with annexin V and am finding that everything is getting labeled. How can I fix this?

Treating cells with trypsin or other reagents to detach adherent cells causes damage to the membrane, such that cells will be labeled with annexin V. The best way to avoid this problem is to allow your cells to recover for 30-45 min in the incubator. Swirl the tube/plate/flask every few minutes to prevent re-attachment. After this recovery period, you can label your cells with annexin V and analyze by flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

Citations & References (14)

Citations & References
Abstract
Regulation of the Fas death pathway by FLICE-inhibitory protein in primary human B cells.
Authors:Hennino A, Berard M, Casamayor-Pallejà M, Krammer PH, Defrance T
Journal:J Immunol
PubMed ID:10975811
'The Fas/Fas ligand (L) system plays an important role in the maintenance of peripheral B cell tolerance and the prevention of misguided T cell help. CD40-derived signals are required to induce Fas expression on virgin B cells and to promote their susceptibility to Fas-mediated apoptosis. In the current study, we ... More
Detection of apoptotic cells by annexin V labeling at electron microscopy.
Authors:Pellicciari C, Bottone MG, Biggiogera M
Journal:Eur J Histochem
PubMed ID:9359032
'Annexin V is a Ca(++)-binding protein which is widely used as a marker for apoptotic cells, as it binds to the phosphatidylserine residues exposed at the surface of apoptotic cells. In this paper, we describe a method for the immunogold-labeling of biotin-conjugated Annexin V, to detect apoptotic thymocytes at electron ... More
Spatiotemporal distribution of dying neurons during early mouse development.
Authors:van den Eijnde SM, Lips J, Boshart L, Vermeij-Keers C, Marani E, Reutelingsperger CP, De Zeeuw CI
Journal:Eur J Neurosci
PubMed ID:10051772
'Apoptosis is a critical cellular event during several stages of neuronal development. Recently, we have shown that biotinylated annexin V detects apoptosis in vivo in various cell lineages of a wide range of species by binding to phosphatidylserines that are exposed at the outer leaflet of the plasma membrane. In ... More
In situ detection of apoptosis during embryogenesis with annexin V: from whole mount to ultrastructure.
Authors:van den Eijnde SM, Luijsterburg AJ, Boshart L, De Zeeuw CI, van Dierendonck JH, Reutelingsperger CP, Vermeij-Keers C
Journal:Cytometry
PubMed ID:9415414
'Apoptosis is of paramount importance during embryonic development. This insight stems from early studies which correlated cell death to normal developmental processes and now has been confirmed by linking aberrant cell death patterns to aberrant development. Linking apoptosis to the phenotype of a developing organism requires spatial information on the ... More
Complement-independent, peroxide-induced antibody lysis of platelets in HIV-1-related immune thrombocytopenia.
Authors:Nardi M, Tomlinson S, Greco MA, Karpatkin S
Journal:Cell
PubMed ID:11551503
'Immunologic thrombocytopenia is seen commonly in HIV-1 infection. The pathogenesis of this problem has been unclear, but it is associated with circulating immune complexes that contain platelet membrane components and anti-platelet membrane GPIIIa49-66 IgG antibodies. These antibodies cause acute thrombocytopenia when injected into mice. We now show that purified anti-GPIIIa49-66 ... More