Citas y referencias (21)

Gibco™

DMEM FluoroBrite™

Name: DMEM FluoroBrite™
SKU: A1896701

El medio Eagle modificado de Dulbecco (DMEM) Gibco™ FluoroBrite™ presenta una fluorescencia de fondo que es comparable a la deMás información

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Número de catálogo	Cantidad
A1896701	500 mL
A1896702	10 x 500 mL

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Número de catálogo A1896701

Precio (MXN)

Cantidad:

500 mL

El medio Eagle modificado de Dulbecco (DMEM) Gibco™ FluoroBrite™ presenta una fluorescencia de fondo que es comparable a la de PBS y un 90 % menor que la emitida por DMEM sin rojo de fenol estándar. Formulado para incluir los nutrientes requeridos para el cultivo celular rutinario cuando se suplementa con suero fetal bovino al 10 % y 4 mM de L-glutamina o el suplemento GlutaMAX™, DMEM FluoroBrite™ está diseñado para mejorar la relación señal-ruido de fluoróforos, lo que permite a los investigadores visualizar incluso los eventos fluorescentes más débiles en un ambiente que favorece una salud celular óptima. Entre las características adicionales se incluyen:

• Mejora de la señal de fluorescencia durante la adquisición de imágenes de células vivas
• Basada en DMEM para ayudar a conservar la salud celular

La microscopía de fluorescencia de células vivas es una técnica esencial para la visualización de eventos biológicos muy importantes y fisiológicamente significativos. Uno de los principales retos que presenta esta técnica es conseguir adquirir imágenes de fluoróforos débiles sin causar daño a la célula, decoloración fotográfica o cambios no deseables en la salud celular. DMEM FluoroBrite™ ayuda a solucionar estos problemas.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Concentración1 X

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Tipo de productoDMEM (medio Eagle modificado de Dulbecco)

Cantidad500 mL

Duración de almacenamiento12 meses a partir de la fecha de fabricación

ClasificaciónLibre de material de origen animal

FormularioLíquido

Serum LevelSuplementos de suero estándar

EsterilidadEstéril con filtro

Con aditivosAlto contenido en glucosa

Sin aditivosSin glutamina, Sin HEPES, Sin rojo fenol, Sin piruvato sódico

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (entre 2 y 8 °C). Proteja el producto de la luz.

Preguntas frecuentes

What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (21)

Citations & References

Abstract

Open source software for quantification of cell migration, protrusions, and fluorescence intensities.

Authors:Barry DJ, Durkin CH, Abella JV, Way M,

Journal:

PubMed ID:25847537

'Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by ... More

A BRCA1-interacting lncRNA regulates homologous recombination.

Authors:Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T,

Journal:

PubMed ID:26412854

Long non-coding RNAs (lncRNAs) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response (DDR). Using a microarray screen, we identify here a novel lncRNA, DDSR1 (DNA damage-sensitive RNA1), which is induced upon DNA damage. DDSR1 induction ... More

Axonal autophagosomes recruit dynein for retrograde transport through fusion with late endosomes.

Authors:Cheng XT, Zhou B, Lin MY, Cai Q, Sheng ZH,

Journal:

PubMed ID:25940348

Efficient degradation of autophagic vacuoles (AVs) via lysosomes is an important cellular homeostatic process. This is particularly challenging for neurons because mature acidic lysosomes are relatively enriched in the soma. Although dynein-driven retrograde transport of AVs was suggested, a fundamental question remains how autophagosomes generated at distal axons acquire dynein ... More

The Nurr1 Activator 1,1-Bis(3'-Indolyl)-1-(p-Chlorophenyl)Methane Blocks Inflammatory Gene Expression in BV-2 Microglial Cells by Inhibiting Nuclear Factor ?B.

Authors:De Miranda BR, Popichak KA, Hammond SL, Jorgensen BA, Phillips AT, Safe S, Tjalkens RB,

Journal:

PubMed ID:25858541

NR4A family orphan nuclear receptors are an important class of transcription factors for development and homeostasis of dopaminergic neurons that also inhibit expression of inflammatory genes in glial cells. The identification of NR4A2 (Nurr1) as a suppressor of nuclear factor ?B (NF-?B)-related neuroinflammatory genes in microglia and astrocytes suggests that ... More

Posttranslational Modification of HOIP Blocks Toll-Like Receptor 4-Mediated Linear-Ubiquitin-Chain Formation.

Authors:Bowman J, Rodgers MA, Shi M, Amatya R, Hostager B, Iwai K, Gao SJ, Jung JU,

Journal:

PubMed ID:26578682

Linear ubiquitination is an atypical posttranslational modification catalyzed by the linear-ubiquitin-chain assembly complex (LUBAC), containing HOIP, HOIL-1L, and Sharpin. LUBAC facilitates NF-?B activation and inflammation upon receptor stimulation by ligating linear ubiquitin chains to critical signaling molecules. Indeed, linear-ubiquitination-dependent signaling is essential to prevent pyogenic bacterial infections that can lead ... More

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