DMEM FluoroBrite™

Citas y referencias (21)

Gibco™

DMEM FluoroBrite™

Name: DMEM FluoroBrite™
SKU: A1896701

El medio Eagle modificado de Dulbecco (DMEM) Gibco™ FluoroBrite™ presenta una fluorescencia de fondo que es comparable a la deMás información

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Número de catálogo	Cantidad
A1896701	500 mL
A1896702	10 x 500 mL

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Número de catálogo A1896701

Precio (MXN)

Cantidad:

500 mL

El medio Eagle modificado de Dulbecco (DMEM) Gibco™ FluoroBrite™ presenta una fluorescencia de fondo que es comparable a la de PBS y un 90 % menor que la emitida por DMEM sin rojo de fenol estándar. Formulado para incluir los nutrientes requeridos para el cultivo celular rutinario cuando se suplementa con suero fetal bovino al 10 % y 4 mM de L-glutamina o el suplemento GlutaMAX™, DMEM FluoroBrite™ está diseñado para mejorar la relación señal-ruido de fluoróforos, lo que permite a los investigadores visualizar incluso los eventos fluorescentes más débiles en un ambiente que favorece una salud celular óptima. Entre las características adicionales se incluyen:

• Mejora de la señal de fluorescencia durante la adquisición de imágenes de células vivas
• Basada en DMEM para ayudar a conservar la salud celular

La microscopía de fluorescencia de células vivas es una técnica esencial para la visualización de eventos biológicos muy importantes y fisiológicamente significativos. Uno de los principales retos que presenta esta técnica es conseguir adquirir imágenes de fluoróforos débiles sin causar daño a la célula, decoloración fotográfica o cambios no deseables en la salud celular. DMEM FluoroBrite™ ayuda a solucionar estos problemas.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Concentración1 X

Calidad de fabricacióncGMP-compliant under the ISO 13485 standard

Tipo de productoDMEM (medio Eagle modificado de Dulbecco)

Cantidad500 mL

Duración de almacenamiento12 meses a partir de la fecha de fabricación

ClasificaciónLibre de material de origen animal

FormularioLíquido

Serum LevelSuplementos de suero estándar

EsterilidadEstéril con filtro

Con aditivosAlto contenido en glucosa

Sin aditivosSin glutamina, Sin HEPES, Sin rojo fenol, Sin piruvato sódico

Unit SizeEach

Contenido y almacenamiento

Almacenar en el refrigerador (entre 2 y 8 °C). Proteja el producto de la luz.

Preguntas frecuentes

What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (21)

Citations & References

Abstract

Open source software for quantification of cell migration, protrusions, and fluorescence intensities.

Authors:Barry DJ, Durkin CH, Abella JV, Way M,

Journal:

PubMed ID:25847537

'Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by ... More

A BRCA1-interacting lncRNA regulates homologous recombination.

Authors:Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T,

Journal:

PubMed ID:26412854

Long non-coding RNAs (lncRNAs) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response (DDR). Using a microarray screen, we identify here a novel lncRNA, DDSR1 (DNA damage-sensitive RNA1), which is induced upon DNA damage. DDSR1 induction ... More

Microtubule-dependent transport and dynamics of vimentin intermediate filaments.

Authors:Hookway C, Ding L, Davidson MW, Rappoport JZ, Danuser G, Gelfand VI,

Journal:

PubMed ID:25717187

We studied two aspects of vimentin intermediate filament dynamics-transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments ... More

Ebola Virus and Severe Acute Respiratory Syndrome Coronavirus Display Late Cell Entry Kinetics: Evidence that Transport to NPC1+ Endolysosomes Is a Rate-Defining Step.

Authors:Mingo RM, Simmons JA, Shoemaker CJ, Nelson EA, Schornberg KL, D'Souza RS, Casanova JE, White JM,

Journal:

PubMed ID:25552710

Ebola virus (EBOV) causes hemorrhagic fevers with high mortality rates. During cellular entry, the virus is internalized by macropinocytosis and trafficked through endosomes until fusion between the viral and an endosomal membrane is triggered, releasing the RNA genome into the cytoplasm. We found that while macropinocytotic uptake of filamentous EBOV ... More

3D imaging of Sox2 enhancer clusters in embryonic stem cells.

Authors:Liu Z, Legant WR, Chen BC, Li L, Grimm JB, Lavis LD, Betzig E, Tjian R,

Journal:

PubMed ID:25537195

Combinatorial cis-regulatory networks encoded in animal genomes represent the foundational gene expression mechanism for directing cell-fate commitment and maintenance of cell identity by transcription factors (TFs). However, the 3D spatial organization of cis-elements and how such sub-nuclear structures influence TF activity remain poorly understood. Here, we combine lattice light-sheet imaging, ... More

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