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Citas y referencias (11)
Invitrogen™
7-Amino-4-Methylcoumarin, reference standard
La 7-amino-4-metilcumarina (AMC) se puede utilizar como estándar de referencia de fluorescencia para sustratos enzimáticos basados en AMC, incluyendo sustratosMás información
| Número de catálogo | Cantidad |
|---|---|
| A191 | 100 mg |
Número de catálogo A191
Precio (MXN)
-
Cantidad:
100 mg
La 7-amino-4-metilcumarina (AMC) se puede utilizar como estándar de referencia de fluorescencia para sustratos enzimáticos basados en AMC, incluyendo sustratos de caspasa basados en AMC. Los espectros de fluorescencia de la AMC no están sujetos a variabilidad debido a la protonación/desprotonación dependiente del pH cuando se someten a un análisis cercano o superior al pH fisiológico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Emisión430 nm
Excitación351 nm
Etiqueta o tinteMetilocumarina
Peso molecular175.2
Tipo de producto7-amino-4-metilocumarina
Cantidad100 mg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaColorantes de cumarina
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Preguntas frecuentes
What solvent should I use to reconstitute 7-Amino-4-Methylcoumarin, reference standard (Cat. No. A191)? Once reconstituted, can the product be aliquoted and stored at -20 degrees C without damaging the molecule?
Citations & References (11)
Citations & References
Abstract
High-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography of glycosphingolipids.
Journal:Anal Biochem
PubMed ID:2327579
'A method combining high-performance liquid affinity chromatography and in situ fluorescent labeling on thin-layer chromatography is introduced for determination of glycosphingolipids. Glycolipids in crude extract from rat liver were separated quantitatively from neutral lipids and phospholipids with a phenylboronic acid-derivatized silica gel column. Glycolipids were eluted quantitatively with approximately 98%
Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay.
Journal:Anal Biochem
PubMed ID:2339787
'A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubation period of between 1 and 16 h, was
New fluorogenic substrates for subtilisin.
Journal:Chem Pharm Bull (Tokyo)
PubMed ID:3899387
A new fluorescent tag for labeling of saccharides.
Journal:Anal Biochem
PubMed ID:6846798
Potential aldehyde groups of N-acetylglucosamine and its di- and tri-saccharides were coupled with 7-amino-4-methylcoumarin (AMC) by reductive amination in the presence of sodium cyanoborohydride. The products were purified by silica gel chromatography. Acid hydrolysis and digestion with glycosidases gave the expected products. One picomole of the sugar-AMC complexes could be
A new fluorogenic substrate for plasmin.
Journal:Biochem J
PubMed ID:161507
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of