Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit
Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit
Citas y referencias (13)
Invitrogen™

Amplex™ Red Xanthine/Xanthine Oxidase Assay Kit

El kit de ensayo de xantina/xantina oxidasa Amplex Red proporciona un método fluorométrico sensible y sencillo para detectar cantidades tanMás información
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Número de catálogoCantidad
A22182400 ensayos
Número de catálogo A22182
Precio (MXN)
-
Cantidad:
400 ensayos
El kit de ensayo de xantina/xantina oxidasa Amplex Red proporciona un método fluorométrico sensible y sencillo para detectar cantidades tan bajas como 200 nM de xantina o hipoxantina o volúmenes tan bajos como 0,1 mU/ml de actividad de xantina oxidasa en un sistema purificado con un volumen de ensayo de 100 µl.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
FormatoTubos, placa de 96 pocillos
Cantidad400 ensayos
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Ensayo de xantina/xantina oxidasa
Para utilizar con (equipo)Lector de microplacas, Espectrofotómetro, Fluorímetro
Línea de productosAmplex
Tipo de productoKit de ensayo de xantina/xantina oxidasa Red
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (13)

Citations & References
Abstract
The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.
Authors:Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C,
Journal:J Endocrinol
PubMed ID:18562629
Endogenous purines including inosine have been shown to exert immunomodulatory and anti-inflammatory effects in a variety of disease models. The dosage of inosine required for protection is very high because of the rapid metabolism of inosine in vivo. The aim of this study was to determine whether a metabolic-resistant purine ... More
Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction.
Authors:Whidden MA, McClung JM, Falk DJ, Hudson MB, Smuder AJ, Nelson WB, Powers SK,
Journal:J Appl Physiol (1985)
PubMed ID:18974366
'Respiratory muscle weakness resulting from both diaphragmatic contractile dysfunction and atrophy has been hypothesized to contribute to the weaning difficulties associated with prolonged mechanical ventilation (MV). While it is clear that oxidative injury contributes to MV-induced diaphragmatic weakness, the source(s) of oxidants in the diaphragm during MV remain unknown. These ... More
Voluntary wheel running restores endothelial function in conduit arteries of old mice: direct evidence for reduced oxidative stress, increased superoxide dismutase activity and down-regulation of NADPH oxidase.
Authors:Durrant JR, Seals DR, Connell ML, Russell MJ, Lawson BR, Folian BJ, Donato AJ, Lesniewski LA,
Journal:J Physiol
PubMed ID:19417091
'Habitual aerobic exercise is associated with enhanced endothelium-dependent dilatation (EDD) in older humans, possibly by increasing nitric oxide bioavailability and reducing oxidative stress. However, the mechanisms involved are incompletely understood. EDD was measured in young (6-8 months) and old (29-32 months) cage control and voluntary wheel running (VR) B6D2F1 mice. ... More
Oxidizing Effects of Exogenous Stressors in Huntington's Disease Knock-in Striatal Cells--Protective Effect of Cystamine and Creatine.
Authors:Ribeiro M, Silva AC, Rodrigues J, Naia L, Rego AC,
Journal:
PubMed ID:24008831
'Huntington''s disease (HD) is a polyglutamine-expansion disease associated to degeneration of striatal and cortical neurons. Previously, we showed that oxidative stress occurs in HD knock-in striatal cells, but little is known regarding cell antioxidant response against exogenous stimuli. Therefore, in the present study we analyzed cellular antioxidant profile following hydrogen ... More
Preserved immune functions and controlled leukocyte oxidative stress in naturally long-lived mice: possible role of nuclear factor kappa B.
Authors:Arranz L, Caamaño JH, Lord JM, De la Fuente M,
Journal:J Gerontol A Biol Sci Med Sci
PubMed ID:20558495
'In order to verify the survival biomarker role of several immune functions, and to determine the oxidation and inflammation mechanisms underlying variability in the aging process, we have investigated a variety of immune functions and oxidative stress parameters as well as activation of the nuclear factor kappa B (NF kappaB) ... More
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