Avidin, NeutrAvidin™, Horseradish Peroxidase conjugate

Nuestro conjugado de estreptavidina-peroxidasa de rábano picante (HRP) NeutrAvidin™ de proteína de unión de biotina (una forma de avidina queMás información

Número de catálogo	Cantidad
A2664	1 mg

Número de catálogo A2664

Precio (MXN)

Cantidad:

Nuestro conjugado de estreptavidina-peroxidasa de rábano picante (HRP) NeutrAvidin™ de proteína de unión de biotina (una forma de avidina que ha sido procesada para eliminar carbohidratos y reducir su punto isoeléctrico) puede disminuir sustancialmente el fondo debido a una unión no específica. El método utilizado para desglicosilar la avidina conserva su unión específica.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Etiqueta o tinteHRP (peroxidasa de rábano picante)

Cantidad1 mg

Condiciones de envíoHielo húmedo

ConjugadoPeroxidasa de rábano picante (HRP)

Línea de productosNeutrAvidin

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador (de -5 a -30 °C).

Citations & References (13)

Citations & References

Abstract

Suppression of nonspecific binding of avidin-biotin complex (ABC) to proteins electroblotted to nitrocellulose paper.

Authors:Clark RK, Tani Y, Damjanov I

Journal:J Histochem Cytochem

PubMed ID:3772081

'Nitrocellulose blots of cell extracts reacted in sequence with biotinylated lectins and horseradish peroxidase-labeled avidin-biotin complex (ABC) often show considerable nonspecific staining of protein bands. Experiments were performed to determine which of the components of the ABC were responsible for this and whether or not the nature and ionic strength ... More

Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton-translocating ATPase.

Authors:Leng XH, Nishi T, Forgac M

Journal:J Biol Chem

PubMed ID:10329659

'The membrane topography of the yeast vacuolar proton-translocating ATPase a subunit (Vph1p) has been investigated using cysteine-scanning mutagenesis. A Cys-less form of Vph1p lacking the seven endogenous cysteines was constructed and shown to have 80% of wild type activity. Single cysteine residues were introduced at 13 sites within the Cys-less ... More

Artifactual detection of biotin on histones by streptavidin.

Authors:Bailey LM, Ivanov RA, Wallace JC, Polyak SW,

Journal:Anal Biochem

PubMed ID:17920026

'Biotinylation is a recent addition to the list of reported posttranslational modifications made to histones. Holocarboxylase synthetase (HCS) and biotinidase have been implicated as biotinylating enzymes. However, the details of the mechanism and the regulation of biotin transfer on and off histones remains unclear. Here we report that in a ... More

Stimulation of the pentose phosphate pathway and glutathione levels by dehydroascorbate, the oxidized form of vitamin C.

Authors:Puskas F, Gergely P, Banki K, Perl A

Journal:FASEB J

PubMed ID:10877828

'Ascorbic acid, or vitamin C, generally functions as an antioxidant by directly reacting with reactive oxygen intermediates and has a vital role in defenses against oxidative stress. However, ascorbic acid also has pro-oxidant properties and may cause apoptosis of lymphoid and myeloid cells. The present study shows that dehydroascorbate, the ... More

Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.

Authors:van Roy N, Mangelschots K, Speleman F

Journal:Trends Genet

PubMed ID:8488564

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