Suero fetal bovino, exosome depleted
Suero fetal bovino, exosome depleted
Gibco™

Suero fetal bovino, exosome depleted

El SBF exosome depleted Gibco agiliza y estandariza los flujos de trabajo de cultivo celular para la investigación de exosomas.Más información
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Número de catálogoCantidadFormato
A2720801500 mLFrasco
A272080350 mLOne Shot
Número de catálogo A2720801
Precio (MXN)
-
Cantidad:
500 mL
Formato:
Frasco
El SBF exosome depleted Gibco agiliza y estandariza los flujos de trabajo de cultivo celular para la investigación de exosomas. Está listo para su uso y el rendimiento probados para ahorrar tiempo y permitir preparaciones de exosomas óptimas.

• Fondo de exosomas más bajo
• Permite resultados uniformes: cada lote probado para el rendimiento del cultivo celular
• No se necesita preparación: no se requiere ultracentrifugación

El SBF con reducción de exosomas se ha reducido en un ≥ 90 % de exosomas endógenos y se ha diseñado para su uso en ensayos de aislamiento de exosomas de cultivo celular. Cada lote se prueba para el rendimiento de cultivo y agotamiento del exosoma, y se realizan nuestros ensayos de control de calidad estándar para ayudar a garantizar resultados óptimos y uniformes. Este SBF listo para su uso para los aislamientos de exosomas de cultivo celular ayuda a eliminar los riesgos asociados a la preparación de reactivos de SBF mediante la ultracentrifugación, para obtener resultados óptimos de ensayos y un mayor rendimiento experimental.

Obtenga más información sobre el SBF con agotamiento de exosomas y su uso en la investigación con exosomas

Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.

Especificaciones
Sustancias contaminantesTriple Filtered at 0.1 μm
Duración de almacenamiento5 Years
Condiciones de envíoCongelado
Tipo especializadoExosome-depleted
EspecieBovine
AgeFetal
País de origenEstados Unidos
FormularioLíquido
FormatoFrasco
Tipo de productoSuero fetal bovino
Cantidad500 mL
Serum TreatmentAgotamiento de exosomas
EsterilidadEstéril
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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