PSC Cardiomyocyte Differentiation Kit
PSC Cardiomyocyte Differentiation Kit
Gibco™

PSC Cardiomyocyte Differentiation Kit

El kit de diferenciación de cardiomiocitos de PSC Gibco™ se compone de un conjunto de medios libres de suero yMás información
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Número de catálogoCantidad
A29212011 kit
Número de catálogo A2921201
Precio (MXN)
-
Cantidad:
1 kit
El kit de diferenciación de cardiomiocitos de PSC Gibco™ se compone de un conjunto de medios libres de suero y xenógenos que permiten una diferenciación eficaz de células madre pluripotentes humanas (PSC) para la contracción de los cardiomiocitos en tan solo 8 días. A diferencia de otros métodos que requieren varios componentes y más duración del ensayo, el kit de diferenciación de cardiomiocitos de PSC puede generar cardiomiocitos a partir de células madre pluripotentes en medios listos para su uso y en menos tiempo.

Compuesto por tres medios 1X que no requieren descongelación ni mezcla, cada medio se emplea de forma consecutiva durante un total de 14 días, lo que da como resultado cardiomiocitos funcionales que expresan marcadores fisiológicos relevantes, se contraen en cultivos y pueden ser posteriormente mantenidos en cultivo durante > 15 días (el medio de mantenimiento también se vende por separado [N.º de cat. A2920801]).

No es necesario descongelar ni mezclar
El kit de diferenciación de cardiomiocitos de PSC se compone de medios 1X que no requieren almacenamiento congelado, así que no hay que descongelar ni mezclar reactivos. Basta calentar los medios y agregarlos al cultivo de PSC.

Producción rápida de cardiomiocitos
El kit de diferenciación de cardiomiocitos de PSC es capaz de producir cardiomiocitos con capacidad de contracción en tan solo 8 días. Los cardiomiocitos diferenciados se pueden mantener posteriormente en cultivo durante > 15 días.

Generación de cardiomiocitos de alta calidad
Los cardiomiocitos generados mediante el kit de diferenciación de cardiomiocitos de PSC son funcionalmente relevantes, ya que expresan marcadores clave como TNNT2, Nkx2.5, MYH6 y α-actinina, al tiempo que se contraen en los cultivos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCardiomiocitos
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Tipo de productoKit de diferenciación de cardiomiocitos de PSC
Unit SizeEach
Contenido y almacenamiento
• 500 ml de medio de mantenimiento de cardiomiocitos
• 100 ml de medio A de diferenciación de cardiomiocitos
• 100 ml de medio B diferenciación de cardiomiocitos

Almacenar entre 2–8 °C y proteger de la luz.

Preguntas frecuentes

Is StemFlex Medium compatible with downstream differentiation kits available from Thermo Fisher Scientific?

Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I used the Gibco PSC Cardiomyocyte Differentiation Kit and am not seeing good differentiation with my iPSC line. What should I do?

We recommend always using H9 or H7 ESC line as a control in your experiments. We recommend adjusting the cell density or extending the induction time for difficult-to-differentiate iPSC lines.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?

Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I maintain differentiated cells in culture using the Gibco PSC Cardiomyocyte Differentiation Kit?

Differentiated cells can be maintained for a month or longer for long-term studies. We recommend the use of Gibco Geltrex Matrix for long-term cultures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which population of cardiomyocytes is produced using the PSC Cardiomyocyte Differentiation Kit?

The population of cardiomyocytes produced is a mix of atrial and ventricular cells. Over time, cultures become more ventricular.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (10)

Citations & References
Abstract
Cardiac disease modeling using induced pluripotent stem cell-derived human cardiomyocytes.
Authors:Dell'Era P, Benzoni P, Crescini E, Valle M, Xia E, Consiglio A, Memo M,
Journal:
PubMed ID:25815118
Causative mutations and variants associated with cardiac diseases have been found in genes encoding cardiac ion channels, accessory proteins, cytoskeletal components, junctional proteins, and signaling molecules. In most cases the functional evaluation of the genetic alteration has been carried out by expressing the mutated proteins in in-vitro heterologous systems. While ... More
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi004-A from a carrier of the KCNQ1-R594Q mutation.
Authors:Mura M, Lee YK, Pisano F, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Tse HF, Gnecchi M
Journal:Stem Cell Res
PubMed ID:30974404
'We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a male carrier of the heterozygous mutation c.1781?G?>?A p.R594Q on the KCNQ1 gene. hiPSCs, generated using four retroviruses each encoding for OCT4, SOX2, KLF4 and cMYC, display pluripotent stem cell characteristics, and can be differentiated into spontaneously beating ... More
Reprogramming of Urine-Derived Renal Epithelial Cells into iPSCs Using srRNA and Consecutive Differentiation into Beating Cardiomyocytes.
Authors:Steinle H, Weber M, Behring A, Mau-Holzmann U, von Ohle C, Popov AF, Schlensak C, Wendel HP, Avci-Adali M
Journal:Mol Ther Nucleic Acids
PubMed ID:31476669
'The generation of induced pluripotent stem cells (iPSCs) from patient''s somatic cells and the subsequent differentiation into desired cell types opens up numerous possibilities in regenerative medicine and tissue engineering. Adult cardiomyocytes have limited self-renewal capacity; thus, the efficient, safe, and clinically applicable generation of autologous cardiomyocytes is of great ... More
Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor.
Authors:Martins GLS, Paredes BD, Sampaio GLA, Nonaka CKV, da Silva KN, Allahdadi KJ, França LSA, Soares MBP, Dos Santos RR, Souza BSF
Journal:Stem Cell Res
PubMed ID:31706097
Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro. ... More
Generation of the human induced pluripotent stem cell (hiPSC) line PSMi007-A from a Long QT Syndrome type 1 patient carrier of two common variants in the NOS1AP gene.
Authors:Mura M, Pisano F, Stefanello M, Ginevrino M, Boni M, Calabrò F, Crotti L, Valente EM, Schwartz PJ, Brink PA, Gnecchi M
Journal:Stem Cell Res
PubMed ID:30878014
We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic Long QT Syndrome (LQTS) type 1 patient, belonging to a South African (SA) founder population segregating the heterozygous mutation c.1022C?>?T p.A341V on the KCNQ1 gene. The patient is also homozygous for the two minor variants rs4657139 and rs16847548 on ... More