Suero fetal bovino, cualificado, Estados Unidos

Name: Suero fetal bovino, cualificado, Estados Unidos
SKU: A3160502

Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información

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Número de catálogo	Cantidad	Formato
A3160502	10 x 50 mL	One Shot
A3160501	50 mL	One Shot
26140079	500 mL	Frasco

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Our improved Premium Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.

Número de catálogo A3160502

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Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Sueros Gibco: Calidad fiable para un rendimiento uniforme

Categoría de los sueros Gibco: Valor plus (rendimiento)
• Uso para cultivo celular general con líneas de células comunes
• Bajos niveles de endotoxina y hemoglobina
• Nivel de endotoxinas: <10 EU/ml
• Nivel de hemoglobina: <25 mg/dl
• Origen: Estados Unidos

El suero Gibco ofrece
• Certificación ISO 13485, procesado en instalaciones registradas de la FDA
• Etiquetado para uso diagnóstico in vitro (conforme con las pruebas de mayor nivel de esterilidad de la Farmacopea Estadounidense [USP])
• Triple filtrado a 0,1 µm
• El frasco Gibco es más fácil de usar en la campana, reduce el riesgo de contaminación y le ayuda a realizar más coherentemente el cultivo celular.
• El adhesivo extraíble de la etiqueta proporciona una referencia práctica del número de lote y la fecha de caducidad en el cuaderno electrónico de laboratorio

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Especificaciones

DescripciónSuero bovino fetal, cualificado, formato One Shot™, Estados Unidos

Concentración de endotoxinas≤10 EU/mL

Concentración de hemoglobina≤25 mg/dL

Línea de productosOne Shot

Grado de pureza o calidadCualificado

Condiciones de envíoCongelado

EspecieBovino

AgeFetal

País de origenEstados Unidos

FormularioLíquido

FormatoOne Shot

Tipo de productoSuero fetal bovino

Cantidad10 x 50 mL

Serum TreatmentEstándar (esterilizado mediante filtro)

EsterilidadEstéril

Sterilization MethodTriple-filtered, 0.1 μm

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

Why would I heat inactivate serum?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat inactivated serum is recommended include immunological studies and culturing ES cells, insect cells, and smooth muscle cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I heat inactivate serum?

Heat at 56 degrees C in a water bath for 30 minutes. Swirl bottle every 10 minutes or so. For accuracy, use a second bottle of similar size and add the equivalent volume of water to this bottle. Place a thermometer in this control bottle in order to see when 56 degrees C is reached. Set your timer for 30 minutes at this point.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the shelf life of Fetal Bovine Serum, qualified, United States once thawed? Can I refreeze it after thawing?

The stability after opening and thawing is application-dependent and most often, it is used within a week or less. The product can be thawed and refrozen. This is standard practice for making aliquots. We recommend that you make smaller aliquots and use as needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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