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Citas y referencias (5)
Invitrogen™
Alexa Fluor™ 647-aha-dCTP
Los colorantes Alexa Fluor® 647, utilizados para etiquetar nucleótidos, son compatibles con los escáneres de micromatrices más utilizados y proporcionanMás información
| Número de catálogo | Cantidad |
|---|---|
| A32771 | 50 μl |
Número de catálogo A32771
Precio (MXN)
-
Cantidad:
50 μl
Los colorantes Alexa Fluor® 647, utilizados para etiquetar nucleótidos, son compatibles con los escáneres de micromatrices más utilizados y proporcionan valores de correlación de señal (R2) mayores que el par de colorantes Cy™3 y Cy™5, lo que mejora la resolución de los ensayos de expresión génica de micromatrices de dos colores a 1,3 cambios de expresión. Los colorantes Alexa Fluor® excepcionalmente brillantes y fotoestables también son esencialmente insensibles al pH y son altamente solubles en agua.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ensayoEnsayo de expresión génica de micromatrices
Concentración1 mM
Método de etiquetadoEtiquetado directo
Etiqueta o tinteColorante Alexa Fluor
ModificaciónAHA (5-aminohexilacrilamido)
Línea de productosAlexa Fluor
Tipo de productoNucleótido etiquetado
Cantidad50 μl
Condiciones de envíoHielo húmedo
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (5)
Citations & References
Abstract
The genetic architecture of Down syndrome phenotypes revealed by high-resolution analysis of human segmental trisomies.
Journal:Proc Natl Acad Sci U S A
PubMed ID:19597142
'Down syndrome (DS), or trisomy 21, is a common disorder associated with several complex clinical phenotypes. Although several hypotheses have been put forward, it is unclear as to whether particular gene loci on chromosome 21 (HSA21) are sufficient to cause DS and its associated features. Here we present a high-resolution
A single-molecule barcoding system using nanoslits for DNA analysis.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17296933
'Molecular confinement offers new routes for arraying large DNA molecules, enabling single-molecule schemes aimed at the acquisition of sequence information. Such schemes can rapidly advance to become platforms capable of genome analysis if elements of a nascent system can be integrated at an early stage of development. Integrated strategies are
Lineage-specific DNA methylation in T cells correlates with histone methylation and enhancer activity.
Journal:Genome Res
PubMed ID:19494038
DNA methylation participates in establishing and maintaining chromatin structures and regulates gene transcription during mammalian development and cellular differentiation. With few exceptions, research thus far has focused on gene promoters, and little is known about the extent, functional relevance, and regulation of cell type-specific DNA methylation at promoter-distal sites. Here,
A single-molecule barcoding system using nanoslits for DNA analysis : nanocoding.
Journal:Methods Mol Biol
PubMed ID:19488691
Single DNA molecule approaches are playing an increasingly central role in the analytical genomic sciences because single molecule techniques intrinsically provide individualized measurements of selected molecules, free from the constraints of bulk techniques, which blindly average noise and mask the presence of minor analyte components. Accordingly, a principal challenge that
Measuring, in solution, multiple-fluorophore labeling by combining fluorescence correlation spectroscopy and photobleaching.
Journal:J Phys Chem B
PubMed ID:20143802
Determining the number of fluorescent entities that are coupled to a given molecule (DNA, protein, etc.) is a key point of numerous biological studies, especially those based on a single molecule approach. Reliable methods are important, in this context, not only to characterize the labeling process but also to quantify