APF (Hydroxyl Radical, Hypochlorite or Peroxynitrite Sensor)
APF (Hydroxyl Radical, Hypochlorite or Peroxynitrite Sensor)
Citas y referencias (17)
Invitrogen™

APF (Hydroxyl Radical, Hypochlorite or Peroxynitrite Sensor)

La aminofenil fluoresceína (APF) y la hidroxifenil fluoresceína (HPF, H36004), dos indicadores de especies reactivas de oxígeno (ROS) desarrollados porMás información
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Número de catálogoCantidad
A36003470 μL
Número de catálogo A36003
Precio (MXN)
-
Cantidad:
470 μL
La aminofenil fluoresceína (APF) y la hidroxifenil fluoresceína (HPF, H36004), dos indicadores de especies reactivas de oxígeno (ROS) desarrollados por T. Nagano, ofrecen mayor especificidad y estabilidad que el diacetato de diclorodihidrofluoresceína (H2DCFDA, D399). La APF y la HPF muestran una reactividad no selectiva limitada y una resistencia relativamente alta a la oxidación inducida por la luz. Estos dos nuevos derivados de la fluoresceína no son fluorescentes hasta que reaccionan con el radical hidroxilo o el anión peroxinitrito; la APF también reacciona con el anión hipoclorito. Juntos, estos dos indicadores ROS pueden detectar selectivamente el anión hipoclorito. Tras la oxidación, tanto la APF como la HPF presentan fluorescencia verde brillante (excitación/emisión máximas ∼490/515 nm), de modo que son compatibles con cualquier instrumento que pueda detectar fluoresceína.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Cantidad470 μL
Tipo de productoROS Indicator
Unit SizeEach

Citations & References (17)

Citations & References
Abstract
Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
Authors:Halliwell B, Whiteman M
Journal:Br J Pharmacol
PubMed ID:15155533
'Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative ... More
Externally applied pressure activates pancreatic stellate cells through the generation of intracellular reactive oxygen species.
Authors:Asaumi H, Watanabe S, Taguchi M, Tashiro M, Otsuki M,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:17761838
'Local tissue pressure is higher in chronic pancreatitis than in the normal pancreas. We reported recently that pressure application induces synthesis of extracellular matrix (ECM) and cytokines in pancreatic stellate cells (PSCs) and that epigallocatechin gallate (EGCG), a potent antioxidant, inhibits the transformation of PSCs from quiescent to activated phenotype ... More
Fluorescence probes used for detection of reactive oxygen species.
Authors:Gomes A, Fernandes E, Lima JL,
Journal:J Biochem Biophys Methods
PubMed ID:16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
Monitoring singlet oxygen and hydroxyl radical formation with fluorescent probes during photodynamic therapy.
Authors:Price M, Reiners JJ, Santiago AM, Kessel D,
Journal:Photochem Photobiol
PubMed ID:19508643
'Singlet oxygen (1O2) is the primary oxidant generated in photodynamic therapy (PDT) protocols involving sensitizers resulting in type II reactions. 1O2 can give rise to additional reactive oxygen species (ROS) such as the hydroxyl radical (*OH). The current study was designed to assess 3''-p-(aminophenyl) fluorescein (APF) and 3''-p-(hydroxyphenyl) fluorescein (HPF) ... More
Myeloperoxidase is a key regulator of oxidative stress mediated apoptosis in myeloid leukemic cells.
Authors:Nakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M,
Journal:Clin Cancer Res
PubMed ID:17875773
'PURPOSE: We reported previously that reactive oxygen species (ROS) are key mediators of apoptosis induced by a polyphenol, (-)-epigallocatechin-3-gallate (EGCG), in myeloid leukemic cells. This study aimed to further examine the mechanism of ROS-mediated apoptosis induced by EGCG and its relationship to the heme enzyme myeloperoxidase (MPO). EXPERIMENTAL DESIGN: We ... More
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