Primary Rat Cortex Neurons, Sprague Dawley
Primary Rat Cortex Neurons, Sprague Dawley
Gibco™

Primary Rat Cortex Neurons, Sprague Dawley

Las neuronas primarias de corteza de rata Gibco, Sprague Dawley, están aisladas y crioconservadas de embriones de ratas Sprague DawleyMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoN.° de celdas
A365111 x 10^6
A365124 x 10^6
Número de catálogo A36511
Precio (MXN)
-
N.° de celdas:
1 x 10^6

Las neuronas primarias de corteza de rata Gibco, Sprague Dawley, están aisladas y crioconservadas de embriones de ratas Sprague Dawley de 18 días. Se trata de una alternativa a las neuronas frescas flexible, lista para usar y de calidad. Cada vial contiene un millón de células por ml. Estas neuronas producen consistentemente una viabilidad posdescongelación de entre el 50 y 80 %, tienen alta pureza neuronal y están validadas para su funcionamiento.

• Alta viabilidad: viabilidad posdescongelación del 50 % o superior
• Alta pureza: fenotipo neuronal superior al 70%
• Validadas para su funcionamiento: se han comprobado en cuanto a flujo de calcio sincrónico espontáneo y actividad de liberación sináptica
 

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
N.° de celdas1 x 10^6
Tipo de productoNeurona de corteza de rata
Cantidad1 x 106 células
Probado paraPureza celular, esterilidad, viabilidad, micoplasma
AgeFetal
Tipo de célulaCélulas neuronales
Culture TypeCultivo de células de mamífero
FormularioCrioconservado
EspecieRata
TissueCorteza
Unit SizeEach
Contenido y almacenamiento
Almacenar en nitrógeno líquido.

Preguntas frecuentes

What is the difference between Primary Rat Cortex Neurons, Sprague Dawley and Primary Rat Cortex Neurons?

These neurons differ in the rat strain from which they were isolated: Sprague Dawley vs. Fisher 344. These neurons don't expand anymore. They just grow neurites. The manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0001574-PrimaryRatCortex-HippocampusNeurons-UG.pdf) has instructions for culturing the cells. You can use Neurobasal Plus/B-27 Plus/GlutaMAX to culture them, or just Neurobasal/B-27/GlutaMAX. However, the former combination provides much superior performance than the latter and can maintain neurons longer.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.

View all Primary Rat Cortex Neurons, Sprague Dawley, 1 x 106 cells FAQs