Search

  • Contáctenos
  • Orden Rápida
Suero fetal bovino, certificado, inactivado por calor, Estados Unidos
Suero fetal bovino, certificado, inactivado por calor, Estados Unidos
Gibco™

Suero fetal bovino, certificado, inactivado por calor, Estados Unidos

Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidadFormato
A384000210 x 50 mLOne Shot
A384000150 mLOne Shot
10082147500 mLFrasco
Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Número de catálogo A3840002
Seleccione el producto actual
Los sueros bovinos fetales Gibco ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto.

Categoría de los sueros: Prémium (rendimiento plus)
Origen: Estados Unidos
Nivel de endotoxinas: ≤5 EU/ml
Nivel de hemoglobina: ≤15 mg/dL
Para uso diagnóstico in vitro.
Especificaciones
DescripciónSuero bovino fetal, inactivado por calor, certificado, One Shot™, Estados Unidos
Concentración de endotoxinas≤5 EU/mL
Inactivado por calorYes
Concentración de hemoglobina≤15 mg/dL
Sustancias contaminantesTriple filtrado a 0,1 μm
Línea de productosOne Shot
Grado de pureza o calidadcontenedores
Duración de almacenamiento5 Years
Condiciones de envíoCongelado
EspecieBovino
AgeFetal
País de origenEstados Unidos
FormularioLiquid
FormatoOne Shot
Tipo de productoSuero fetal bovino
Cantidad10 x 50 mL
Serum TreatmentInactivado por calor
EsterilidadEstéril
Sterilization MethodTriple filtrado, 0,1 μm
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Suero fetal bovino, certificado, inactivado por calor, Estados Unidos FAQs