Expi293™ PRO Expression Medium

Gibco Expi293 PRO Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293 PRO cells in the Expi293 PRO Expression System for scalable transient protein expression.

• Supports growth of suspension HEK 293 cultures to densities of over 15 x 10^6 cells/mL
• Transfection-compatible serum-free suspension culture medium
• Helps enable sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 5 g/L of recombinant protein
• Supports growth and transfection of HEK293 cells in culture formats of 1 mL to 10 L
• Does not contain phenol red

Expi293 PRO Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 PRO Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.