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CTS™ Opti-MEM™ I Medium
Gibco™

CTS™ Opti-MEM™ I Medium

Opti-MEM™ I CTS™ es un MEM mejorado que se recomienda para usar con el kit de transfección LV-MAX™ CTS™ como tampón de formación de complejos.
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Número de catálogoCantidad
A4124801100 mL
A4124802500 mL
Número de catálogo A4124801
Precio (MXN)
904.79
Each
Cantidad:
100 mL
Precio (MXN)
904.79
Each

Gibco™ CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTS LV-MAX Transfection Kit as a complexation buffer. This medium contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. CTS Opti-MEM I uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. The formulation of CTS Opti-MEM I is identical to research-grade Opti-MEM I Reduced Serum Medium.

CTS Opti-MEM I is xeno-free and contains human transferrin.

CTS Opti-MEM I is manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

Custom configuration and packaging available upon request.

El medio Opti-MEM I CTS Gibco es un medio esencial mínimo (MEM) mejorado que se recomienda para su uso con el kit de transfección LV-MAX CTS como tampón de formación de complejos. Este medio contiene insulina, transferrina, hipoxantina, timidina y elementos traza. Opti-MEM I CTS utiliza un sistema de tampones de bicarbonato sódico (2,4 g/l) y, por lo tanto, requiere un ambiente con un 5–10 % de CO2 para mantener el pH fisiológico. La formulación es idéntica al medio de suero reducido Opti-MEM I con calidad para investigación.

Opti-MEM I CTS no contiene componentes xenobióticos, pero sí transferrina humana.

Opti-MEM I CTS se elabora según las buenas prácticas de fabricación actuales para dispositivos médicos, 21 CFR, Parte 820, y se ha certificado según la ISO 13485. El archivo de asistencia normativa está disponible previa solicitud.

Configuración personalizada y embalaje disponibles previa solicitud.

Para su uso en investigación o fabricación de productos basados en células, genes o tejidos. PRECAUCIÓN: no diseñado para la administración directa en seres humanos o animales.
Especificaciones
Línea de célulasViral Production Cells
Tipo de célulaHEK293
Para utilizar con (aplicación)LV Production
FormatoFrasco
Calidad de fabricaciónISO 13485, MDSAP, FDA-registered, 21 CFR 820
Tipo de productoMedio esencial mínimo (MEM)
Cantidad100 mL
Duración de almacenamiento18 months from date of manufacture
Condiciones de envíoAmbiente
ClasificaciónAnimal Origin-free
FormularioLíquido
EsterilidadEstéril con filtro
Unit SizeEach
Contenido y almacenamiento
Almacenar a entre 2 y 8 °C y proteger de la luz

Preguntas frecuentes

Why do you recommend using V bottom 96-well plates for harvesting lentivirus from CTS Viral Production Cells?

We recommend using V-bottom 96-well plates to harvest the supernatant containing lentiviral particles as they allow for easier detection of the cell pellets after centrifugation of the cultures.

During lentivirus production from CTS Viral Production Cells, will the shaker vibration affect the cell growth of other cultures maintained in the same incubator or in stacked incubators?

We recommend maintaining shaking cultures in a separate incubator from all other incubators to avoid vibration effects. Alternatively, we recommend using a small, quiet shaker such as the Thermo Scientific CO2 Resistant Shaker (Cat. No. 88881102) for convenience and best performance.

Is 8% CO2 a growth requirement for CTS Viral Production Cells?

We generally recommend culturing the CTS Viral Production Cells under 8% CO2 conditions. We found that cells cultured in 5% CO2 grew slower and were more prone to more cell clumping than when grown in 8% CO2.

What is special about the CTS LV-MAX lentivirus production system?

The CTS LV-MAX lentivirus production system is a fully-optimized complete system in which the cells, media, and transfection reagent work together to generate high-titer lentivirus. The CTS LV-MAX Production Medium is specifically designed to work with the system, to support high density suspension cell growth of CTS Viral Production Cells.

The manual recommends using CTS Viral Production Cells passaged to P5 (˜3 weeks) on the day of transfection. Are these general cell culture guidelines or special requirements for these cells?

Although 3-4 passages before transfection are sufficient for most cell lines recovering directly from liquid N2 storage, we found that CTS Virus Production Cells passaged to P5 showed the highest performance in lentivirus particle production and reproducibility between batches. Therefore, we recommend passaging the cells to P4 at the end of a given week for lentivirus production the following week.

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