Are there larger volume standalone reagent components available to scale up the MagMAX HMW DNA workflow?
Yes, the following larger volume standalone components are available for purchase separately:
- MagMAX HMW DNA Booster, 1,000 preps (1.0 mL) (Cat. No. A36625)
- MagMAX DNA Multi-Sample Ultra 2.0 Proteinase K (45 mL) (Cat. No. A36578)
- MagMAX DNA Multi-Sample Ultra 2.0 Binding Beads (45 mL) (Cat. No. A36579)
- MagMAX DNA Multi-Sample Ultra 2.0 Wash I Solution (1.1 L) (Cat. No. A36580)
- MagMAX DNA Multi-Sample Ultra 2.0 Lysis Binding Solution (450 mL) (Cat No. A36581)
- MagMAX DNA Multi-Sample Ultra 2.0 Elution Solution (120 mL) (Cat No. A36582)
- MagMAX DNA Multi-Sample Ultra 2.0 Enhancer Solution (45 mL) (Cat No. A36583)
- MagMAX Cell and Tissue DNA Extraction Buffer (600 mL) (Cat No. A45470)
If I have already purchased the MagMAX DNA Multi-Sample Ultra 2.0 Kit or standalone components, what do I need to extract high molecular weight DNA?
In addition to the MagMAX DNA Mult-Sample Ultra 2.0 product(s) you will need:
MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624) or
In addition to the MagMAX DNA Mult-Sample Ultra 2.0 product(s) you will need:
MagMAX HMW DNA Booster, 100 preps (Cat. No. A36624) or MagMAX HMW DNA Booster, 1,000 preps (Cat No. A36625).
For cell and tissue samples include:
MagMAX Cell and Tissue DNA Extraction Buffer, 100 preps (Cat. No. A45469) or MagMAX Cell and Tissue DNA Extraction Buffer, 1,000 preps (Cat. No. A45470)
The MagMAX HMW DNA User Guide (MAN1001478)
The specific HMW DNA KingFisher automation protocol for your KingFisher Purification System (see product description page for download).
MagMAX HMW DNA Booster, 1,000 preps (Cat No. A36625).
For cell and tissue samples include:
MagMAX Cell and Tissue DNA Extraction Buffer, 100 preps (Cat. No. A45469) or MagMAX Cell and Tissue DNA Extraction Buffer, 1,000 preps (Cat. No. A45470)
The MagMAX HMW DNA User Guide (MAN1001478)
The specific HMW DNA KingFisher automation protocol for your KingFisher Purification System (see product description page for download).
Can the MagMAX HMW DNA Booster be paired with other genomic DNA isolation kits?
The MagMAX HMW DNA Booster is specifically designed and optimized for use with the MagMAX DNA Multi-Sample Ultra 2.0 (Cat. No. A36570) chemistry.
Can the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) be used to isolate genomic DNA from bone marrow aspirates and PBMCs?
Yes, we have protocols for using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) to isolate genomic DNA from these sample types, but they require purchase of an additional Cell and Tissue Extraction Buffer. The kit and buffer can be purchased together as Cat. No. A45721 or the Cell and Tissue Extraction Buffer can be purchased as a stand alone item (Cat. No. A45469 or A45470).
The suggested workflow for bone marrow aspirates is as follows:
- Use only up to 200 µL of bone marrow aspirate
- Centrifuge at 200 x g for 10 mins
- Remove the supernatant (leaving about 50 µL of aspirate with the loose pellet) and resuspend the cell pellet in 400 µL of Cell and Tissue Extraction Buffer
- From this point, follow the protocol for isolation of DNA from buffy coat samples
The suggested workflow for 1-2.0 x 10E6 PBMCs is as follows:
- Centrifuge at 500-800 x g for 10 mins
- Remove the supernatant (leaving the cell pellet) an resuspend the cell pellet in 400 L of Cell and Tissue Extraction Buffer
- From this point, follow the protocol for isolation of DNA from cultured cell samples
Can the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) be used to isolate DNA from cultured cells and tissue?
Yes, we have protocols for using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) to isolate DNA from cells and tissue, but they require purchase of an additional Cell and Tissue Extraction Buffer. The kit and buffer can be purchased together as Cat. No. A45721 or the Cell and Tissue Extraction Buffer can be purchased as a stand alone item (Cat. No. A45469 or A45470).
