Oncomine™ Myeloid Assay GX v2

The assay kit includes the following components sufficient for up to 32 x 3-pool reactions (32 x 2-pool DNA reactions and 32 x 1-pool RNA reactions):

Myeloid DNA Pool 1 v2 (position 1)
2 packs of 4 tubes each, 140 ul per tube, magenta carrier color, 2 x 16 reactions (4 reactions/tube) stored at-30C to -10C

Myeloid DNA Pool 2 v2 (position 1)
2 packs of 4 tubes each, 140 ul per tube, pale green carrier color, 2 x 16 reactions (4 reactions/tube) stored at -30C to -10C

Myeloid RNA Pool 1 v2 (position 1)
1 pack of 8 tubes, 70 ul per tube, pale orange carrier color, 32 reactions (4 reactions/tube) stored at -30C to -10C

Genexus Library Strips 1 and 2-AS
3 packs of 8 strips each (total 24 strips of each type); Strip 1 stored at 2C to 8C, Strip 2-AS stored at -30C to -10C

The Oncomine Myeloid Assay GX v2 kit includes DNA and RNA panel components configured for up to 32 reactions. The kit contains:
• DNA panels provided in two pools across multiple tubes
• RNA panel provided as a single pool
• Genexus Library Strips for automated processing These components support both DNA and RNA workflows within a single kit.

No, the Oncomine Myeloid Assay GX v2 kit (Cat. No. A50694) is offered as a complete kit for 32 samples. Individual panel components are not available as standalone products.

Remove the templating strips from their boxes in the refrigerator or freezer and get them ready them for loading in the sequencer by equilibrating to/thawing at room temperature for 30 mins. Confirm that the strip contents are completely thawed before loading in the sequencer. If you are delayed in loading, keep the thawed strips on ice or at 4 degrees C until you load them in the sequencer.

Genexus Strip 3B‑GX5 contain magnetic beads in one or two positions, yellow or brown in color, that sometimes get trapped in the upper “keyhole” of the tube. Dislodge these beads from the keyhole before installing the strip in the sequencer using the following procedure for each strip:
- Invert the strip 3-4 times to dislodge beads that are trapped in the keyholes.
- To remove any remaining beads and liquid from the keyholes, grasp the strip at one end with the strip seal facing up, then swing the strip with a rapid, downward centrifugal arm motion, ending with a sharp wrist-flick.
- Grasp the strip at the other end, then repeat the centrifugal motion.
- Check tube positions for significant amounts of beads that are still trapped in keyholes and then repeat the centrifugal motion, if needed. It is acceptable if a few beads remain in the keyhole or on the tube wall, but most should be either in suspension or in a pellet at the bottom of the tube.

Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) has new Genexus Strip 3B that has the chemistry modified to improve templating and sequencing of GC rich regions.