Proteína A de alta capacidad Pierce™ MagBeads, alcalino estable
Proteína A de alta capacidad Pierce™ MagBeads, alcalino estable
Thermo Scientific™

Proteína A de alta capacidad Pierce™ MagBeads, alcalino estable

La proteína A de alta capacidad Pierce MagBeads, alaclino estable, es una partícula magnética de alto rendimiento para purificar anticuerpos del sobrenadante de cultivo celular, ascitis, suero y extractos crudos.
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Número de catálogoCantidad
A530365 mL
A530351 mL
A5303725 mL
A530384 x 25 mL
Número de catálogo A53036
Precio (MXN)
-
Cantidad:
5 mL

Pierce High-Capacity Protein A MagBeads, Alkali Stable, are high-performance magnetic beads for purification of antibodies from cell culture supernatant, ascites, serum, and crude extracts.

Features of the High-Capacity Protein A MagBeads include:

  • High binding capacity—≥ 40 mg HIgG/mL settled beads
  • Alkaline stable—engineered to withstand standard CIP procedures with 0.1 M NaOH, enabling efficient cleaning of the beads for reuse
  • Animal component-free raw materials
  • Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)
  • Stable—high-affinity Protein A is securely immobilized to magnetic agarose to help prevent leaching of ligand, target antibody, or base support

The protein A ligand has been genetically modified for stability in the presence of NaOH, so the beads can be regenerated at least 10 times without sacrificing binding capacity. The ligand is produced in yeast and is immobilized on magnetic agarose with an average particle size of ∼30 μm.

Pierce High-Capacity Protein A MagBeads, Alkali-Stable, are suitable for purifying mAbs and a subpopulation of scFv's and Fab fragments containing a VH3 domain. The beads are compatible with automation, allowing for higher throughput purifications and screening of purification conditions.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Volumen del lecho1.25 mL
Capacidad de unión≥ 40 mg de HIgG/ml de gránulos asentados
ConcentraciónSlurry: 25%
DescripciónGránulos magnéticos de agarosa de alta capacidad
Para utilizar con (aplicación)Purificación de anticuerpos
FormatoFrasco
Tipo de ligandoProteína A de alcalina estable
Cantidad5 mL
Capacidad (métrico)5 mL
Tamaño de partículas∼ 30 μm
Unit SizeEach
Contenido y almacenamiento
Se suministra en forma de una pulpa del 25 %.

Tras la recepción, almacenar a 2–8 °C.

Preguntas frecuentes

Can I automate the workflow using Pierce High Capacity Protein A MagBeads, alkali stable?

Yes. A KingFisher Apex protocol for 96-deep well plates is provided on pages 3 and 4 in the instruction manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0026311_Pierce_HiCap_proteinA_MagBeads_UG.pdf).

Using Pierce High Capacity Protein A MagBeads, alkali stable, what downstream assays are compatible with the eluted samples?

Purified samples eluted from Pierce High Capacity Protein A MagBeads, alkali stable are compatible with protein assays (Bradford, BCA, Rapid Gold, A280, etc.) and can be used for industry standard testing applications (western blotting, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, etc.).

After regeneration of Pierce High Capacity Protein A MagBeads, alkali stable, is there a specific buffer in which the beads should be stored?

If the beads are not going to be re-used immediately, we recommend using 20% ethanol for long-term storage.

How do I regenerate Pierce High Capacity Protein A MagBeads, alkali stable?

Using the Clean-In-Place (CIP) protocol provided in the instruction manual, we recommend cleaning the Pierce High Capacity Protein A MagBeads, alkali stable with 20 bed volumes of 0.1 M NaOH after every run.

How many uses/regenerations are possible with Pierce High Capacity Protein A MagBeads, alkali stable?

Pierce High Capacity Protein A MagBeads, alkali stable can be reused at least 10 times with <5% loss in binding capacity.