Lyo-ready Bst DNA Polymerase
Lyo-ready Bst DNA Polymerase
Invitrogen™

Lyo-ready Bst DNA Polymerase

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
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Número de catálogoCantidadConcentración
A566571200 U40 U/μL
A566551200 U6 U/μL
A566566000 U6 U/μL
Número de catálogo A56657
Precio (MXN)
-
Cantidad:
1200 U
Concentración:
40 U/μL
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP).

Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity. It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies.

Lyo-ready Bst DNA Polymerase features
• Fast target detection in as little as 10 minutes
• Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction
• Robust amplification, even from inhibitor-containing RNA or DNA samples
• Ability to optimize LAMP or RT-LAMP reactions to fit your needs

Applications
Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Recombinase polymerase amplification (RPA)
• Helicase-dependent amplification (HDA)
• Exponential amplification reaction (EXPAR)

Notes
1. If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use.
2. SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración40 U/μL
N.º de reacciones200 Reactions
Temperatura óptima de reacción65°C
Tiempo de reacción≤10 minutes
Condiciones de envíoDry Ice
Para utilizar con (aplicación)LAMP, RT-LAMP
PolimerasaLyo-ready Bst DNA Polymerase
Tipo de productoStand-alone enzyme
Cantidad1200 U
Unit SizeEach
Contenido y almacenamiento

• Lyo-ready Bst DNA Polymerase (30 μL at 40 U/μL)
• 10X Bst Reaction Buffer (1 mL)
• 200 mM MgCl2 (1 mL)
• 5 M Betaine (1 mL)

Store at –15 to –25°C.

Preguntas frecuentes

Is it possible to combine an (RT-)LAMP reaction with colorimetric detection methods using Lyo-ready Bst DNA polymerase?

Yes, LAMP can be combined with colorimetric detection using calcein. We recommend adding calcein and MnCl₂ to the reaction mixture at final concentrations of 80 µM and 0.25 mM, respectively. Prior to amplification, calcein binds to Mn²⁺ ions, giving the mixture an orange color. During amplification, Mn²⁺ ions form a complex with pyrophosphate, releasing calcein and causing the reaction mixture to change to a green or yellowish-green color, indicating successful amplification.

How is 1 unit of enzyme activity defined for Lyo-ready Bst DNA Polymerase?

One unit of enzyme activity is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into a polynucleotide fraction at 65 degrees C in 30 min.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Does Lyo-ready Bst DNA Polymerase incorporate dUTP?

Lyo-ready Bst DNA Polymerase has the ability to incorporate dUTP during amplicon generation. Use of 100% of dUTP instead of dTTP in the dNTP mix can lead to slower target detection.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

I noticed that my Lyo-ready Bst DNA Polymerase is frozen after storage at -20 degrees C. Is it still good to use?

Lyo-ready Bst DNA Polymerase comes in a storage buffer that does not contain glycerol. Product freezing doesn't affect its performance or stability. We do not recommend freeze-thawing Lyo-ready Bst DNA Polymerase for more than 20 cycles. Lyo-ready Bst DNA Polymerase is stable for 24 months when stored at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Do you offer Lyo-ready Bst DNA Polymerase in lyophilized/dry format?

No. However, Lyo-ready Bst DNA Polymerase comes in a glycerol-free formulation that can be directly air-dried or lyophilized. Dry formulation maintains stability and shows good performance after reconstitution in 10X Bst Reaction Buffer. Read more about lyophilization and air-drying of Lyo-ready Bst DNA Polymerase in the application note “Assessing Lyo-ready Bst DNA Polymerase Stability after Air-drying and Lyophilization” on thermofisher.com/lamp.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all Lyo-ready Bst DNA Polymerase FAQs