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Citas y referencias (33)
Invitrogen™
ATTO-TAG™ CBQCA Derivatization Reagent (CBQCA; 3-(4-Carboxybenzoyl)quinoline-2-Carboxaldehyde)
El reactivo de derivatización ATTO-TAG™ CBQCA (CBQCA; 3-(4carboxibenzoilo)quinolina- 2-carboxaldehído) reacciona específicamente con aminas primarias para formar conjugados que pueden analizarseMás información
| Número de catálogo | Cantidad |
|---|---|
| A6222 también denominado A-6222 | 10 mg |
Número de catálogo A6222
también denominado A-6222
Precio (MXN)
-
Cantidad:
10 mg
El reactivo de derivatización ATTO-TAG™ CBQCA (CBQCA; 3-(4carboxibenzoilo)quinolina- 2-carboxaldehído) reacciona específicamente con aminas primarias para formar conjugados que pueden analizarse por métodos electrofórticos o cromatográficos. Los productos resultantes de CBQCA tienen un máximo de excitación a 450 nm o por la línea espectral 442 nm del láser de He-Cd y cuentan con una emisión máxima de ∼ 550 nm. En la electroforesis de zona capilar (CZE), la sensibilidad de detección de conjugados ATTO-TAG™ CBQCA debe estar a nivel de atomoles (10-18 moles) La alta sensibilidad, la no necesidad de fondo y la excitabilidad de longitud de onda larga hacen que estos reactivos potenciales sean ideales para aplicaciones forenses, de investigación y de diagnóstico.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Reactividad químicaAmina
Para utilizar con (aplicación)Viabilidad celular, proliferación y función; electroforesis de proteínas y Western blot
Etiqueta o tinteATTO-TAG CBQCA
Cantidad10 mg
Fracción reactivaAldehído
Condiciones de envíoTemperatura ambiente
ObjetivoAmina primaria
FormularioMaciza
Línea de productosATTO-TAG
TipoReactivo de derivatización
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de –5 a –30 °C) y proteger de la luz.
Preguntas frecuentes
Can CBQCA (3-(4carboxybenzoyl) quinoline- 2-carboxaldehyde) be used to label reducing sugars?
Can NaCN be used instead of KCN in the CBQCA (3-(4carboxybenzoyl) quinoline- 2-carboxaldehyde) assay?
Citations & References (33)
Citations & References
Abstract
CBQCA assay of primary amine losses during hemodialysis.
Journal:Clin Chim Acta
PubMed ID:11412827
Nitrogen loss during hemodialysis is an important issue since all steps taken to improve clearance of urea will also increase loss of amino acids and other normally recyclable sources of nitrogen. Most dialyzers exclude albumin and other proteins, which react well with dyes commonly employed to measure protein, but do
Derivatization in capillary electrophoresis.
Journal:J Chromatogr A
PubMed ID:9646486
'In recent years capillary electrophoresis (CE) has been developed into a versatile separation technique, next to gas and liquid chromatography (LC), well suited for the determination of a wide variety of e.g., pharmaceutical, biomedical and environmental samples. The main advantages of CE over chromatographic separation techniques are its simplicity and
Capillary electrophoresis with laser-induced fluorescence detection: a sensitive method for monitoring extracellular concentrations of amino acids in the periaqueductal grey matter.
Journal:J Neurosci Methods
PubMed ID:8815306
'The use of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the analysis of microdialysate samples from the periaqueductal grey matter (PAG) of freely moving rats is described. By employing 3-(4-carboxybenzoyl)-2-quinoline-carboxaldehyde (CBQCA) as a derivatization agent, we simultaneously monitored the concentrations of 8 amino acids (arginine, glutamine, valine, gamma-amino-n-butyric acid
Mycothiol biosynthesis and metabolism. Cellular levels of potential intermediates in the biosynthesis and degradation of mycothiol in mycobacterium smegmatis.
Journal:J Biol Chem
PubMed ID:9804803
'Mycothiol (MSH; 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-alpha-D- glucop yranoside (AcCys-GlcN-Ins)) is a novel thiol produced at millimolar levels by mycobacteria and other actinomycetes that do not make glutathione. We developed methods to determine the major components of MSH (AcCys, Cys-GlcN, AcCys-GlcN, Cys-GlcN-Ins, GlcN-Ins) in cell extracts. Mycobacterium smegmatis was shown to produce measurable levels
Identification of a recombinant synaptobrevin-thioredoxin fusion protein by capillary zone electrophoresis using laser-induced fluorescence detection.
Journal:J Chromatogr B Biomed Sci Appl
PubMed ID:9271130
'Capillary zone electrophoresis (CZE) was utilized to identify a synaptobrevin-thioredoxin fusion protein (TSB-51). TSB-51 is a substrate for cleavage by botulinum toxin B at the Q(76)-F(77) site. TSB-51 was derivatized with a fluorophore, CBQCA [3-(4-carboxy-benzoyl)-2-quinoline-carboxaldehyde], for 4 h at room temperature. Optimal conditions for CZE separation of the TSB-51-CBQCA complex