Residual Protein TrypLE™ ELISA Kit
Residual Protein TrypLE™ ELISA Kit
Gibco™

Residual Protein TrypLE™ ELISA Kit

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLEMás información
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Número de catálogoCantidad
A6533103960 pruebas
A653310196 pruebas
A65331025 kits, 480 tests
Número de catálogo A6533103
Precio (MXN)
-
Cantidad:
960 pruebas

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.

TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.

Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.

Especificaciones
Intervalo del ensayo0.78 to 50 ng/mL
Sensibilidad del ensayo0.032 ng/mL
ConjugadoHorseradish Peroxidase (HRP)
Para utilizar con (equipo)Lector de microplacas colorimétrico
FormatoWash steps required
CV interensayo6.20%
CV intraensayo7.20%
Etiqueta o tinteHorseradish Peroxidase (HRP)
Alias de proteínaTrypLE, R-Protease
Cantidad960 pruebas
Condiciones de envíoWet Ice
ObjetivoTrypLE (R-Protease) concentration
Tiempo total del ensayo1.5 hr.
Formato del kit de inmunoensayoSandwich ELISA Kit
Unit SizeEach
Contenido y almacenamiento
• Pre-coated 96-well plate
• Standard assay buffer concentrate
• HRP-conjugated detection antibody
• Wash buffer concentrate
• Chromogen stop solution
• Adhesive plate covers

Store at 2–8°C.

Preguntas frecuentes

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.