Cy3™ Dye-Labeled Anti-miR™ Negative Control #1

Inhibidor de ARNmi sintético etiquetado con colorante Ambion™ Cy™3 para el análisis funcional de ARNmi. Se suministra en cantidad deMás información

Número de catálogo	Cantidad
AM17011	5 nmol

Número de catálogo AM17011

Precio (MXN)

Cantidad:

5 nmol

Inhibidor de ARNmi sintético etiquetado con colorante Ambion™ Cy™3 para el análisis funcional de ARNmi. Se suministra en cantidad de 5 nmol, purificado y listo para transfeccionar. Los inhibidores de miARN Ambion™ Anti-miR™ son ácidos nucleicos monocatenarios modificados químicamente diseñados para unirse e inhibir específicamente las moléculas endógenas de microARN (miARN). Estos inhibidores listos para su uso pueden introducirse en las células mediante parámetros de transfección o electroporación similares a los utilizados para los ARNip. El control negativo #1 etiquetado con colorante Anti-miR™ Cy™3 es una molécula mimetizadora de miARN de secuencia aleatoria que se ha sometido a numerosas pruebas con líneas de células y tejidos humanos y se ha validado que no produce efectos identificables en la función conocida del miARN. Se utiliza para supervisar la eficacia de la transfección en experimentos de transfección utilizando inhibidores de miARN Anti-miR. La etiqueta fluorescente permite la observación directa de la captación celular, la distribución y la localización del control. La molécula está etiquetada en su extremo 5' y tiene la misma secuencia de oligonucleótidos que el control negativo inhibidor de miARN Anti-miR™ (SKU n.º AM17010).

Productos accesorios:
También están disponibles los inhibidores de miARN Anti-miR™ (SKU n.º AM17000 y n.º AM17001). Para seleccionar su secuencia y orden de miARN, por favor visite GeneAssist™ miRNA Workflow Builder. Hay disponibles controles negativos etiquetados con colorante Anti-miR™ FAM™ (SKU #AM17012). El kit de inicio de miARN Pre-Mir™ (SKU n.º AM1540) se puede utilizar para optimizar la transfección de los precursores de miARN Pre-Mir™.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Análisis de ARN sin codificación y Mirna

Etiqueta o tinteCy3

Línea de productosAmbion™, Anti-miR™, Cy™3

Tipo de productoInhibidor de miARN

Cantidad5 nmol

Condiciones de envíoHielo seco

Tipo de controlControl negativo

RNAi TypeInhibidor de miARN

EspecieHumano, ratón, rata

Unit SizeEach

Contenido y almacenamiento

El miARN anti-Mir™ se suministra secado en un solo tubo, junto con agua sin nucleasas para resuspensión, y debe almacenarse a – 20 °C. Uso garantizado de 1 año cuando se almacena seco a –20 °C.

Preguntas frecuentes

Where can I find more information about published miRNA sequences and annotation?

Please visit miRBase: The microRNA database (http://www.mirbase.org/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I would like to order a mimic or inhibitor with a 5' label. Can you do this?

We can label our Pre-miR and Anti-miR miRNA, but do not offer labeled mirVana Mimics and inhibitors. 5' modifications include Alexa Fluor 555, Alexa Fluor 647, FAM, and Cy3. Request a quote here (http://www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

The miRNA sequence I need to target is not in the miRBase database. Can I order a custom mimic or inhibitor?

Yes, submit your unpublished miRNA sequences and/or order custom modified miRNA here (http://www.thermofisher.com/order/custom-genomic-products/tools/mirna/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the difference between Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors and mirVana miRNA mimics and inhibitors?

Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors are our first generation mimic and inhibitors. Invitrogen Anti-miR miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind and inhibit endogenous microRNA (miRNA) molecules. These are ready to use inhibitors that can be introduced into cells using transfection or electroporation. Pre-designed inhibitors have been created for all miRNAs listed in miRBase version 15.0.
Invitrogen Pre-miR miRNA Precursors are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Please note, Pre-miR miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
mirVana miRNA mimics and inhibitors are our second generation of mimics and inhibitors. mirVana miRNA mimics are small, chemically double-stranded RNAs that mimic endogenous mature miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. They have enhanced specificity over the first generation mimics, are delivered as HPLC purified, and ready to transfect.
miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity. They have enhanced potency over our first generation inhibitors. Pre-designed inhibitors were created for all miRNAs listed in miRBase version 19.0. They come delivered as HPLC purified, and ready to transfect. Learn more about mirVana miRNA mimics and inhibitors through this video (https://www.youtube.com/watch?feature=player_embedded&v=Q3MO1lmT0kw). Both mimics and inhibitors are available individually or as libraries.
They are compatible with both in vitro and in vivo applications, and have been validated with Lipofectamine RNAiMAX Transfection Reagent for use in cell-based systems, and with Invivofectamine 3.0 Transfection Reagent for in vivo delivery. These oligonucleotides are nontoxic and do not induce an immune response in the animal models tested. In vivo-ready mirVana miRNA mimics and inhibitors have been purified by HPLC and dialysis and are ready for immediate in vivo use. For all library inquiries, please email: rnailibraries@lifetech.com.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the difference between siRNA and miRNA?

siRNA mediates degradation of a target mRNA molecule in a sequence-specific manner, while miRNA suppresses translation without cleaving mRNA.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all Cy3™ Dye-Labeled Anti-miR™ Negative Control #1 FAQs