Citas y referencias (21)

Invitrogen™

Phalloidin Labeling Probes

Name: Phalloidin Labeling Probes
SKU: B3475

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.

Cambiar vista

Número de catálogo	Color	Intervalo de longitud de onda de excitación	Tipo de colorante
B3475	Rojo	558⁄569	BODIPY™
A22281	Azul	346⁄442	Alexa Fluor™ 350
A30104	Violeta	405/450	Alexa Fluor™ Plus 405
A12379	Verde	495⁄518	Alexa Fluor™ 488
O7466	Verde	496⁄520	Oregon Green™ 488
F432	Verde	496⁄516	FITC (fluoresceína)
A22282	Amarillo	531⁄554	Alexa Fluor™ 532
R415	Rojo anaranjado	540⁄565	TRITC (isotiocianato de tetrametilrodamina)
A22283	Naranja	556⁄570	Alexa Fluor™ 546
A34055	Naranja	555⁄565	Alexa Fluor™ 555
A30106	Naranja	555/565 nm	Alexa Fluor Plus 555
A12380	Rojo anaranjado	578⁄600	Alexa Fluor™ 568
A12381	Rojo	581⁄609	Alexa Fluor™ 594
T7471	Rojo	591⁄608	Texas Red™
A22284	Rojo lejano	632⁄647	Alexa Fluor™ 633
A34054	Rojo lejano	633⁄647	Alexa Fluor™ 635
A22287	Rojo lejano	650⁄668	Alexa Fluor™ 647
A30107	Rojo lejano	650/668 nm	Alexa Fluor Plus 647
A22285	Infrarrojo cercano	663⁄690	Alexa Fluor™ 660
A22286	Infrarrojo cercano	679⁄702	Alexa Fluor™ 680
A30105	Infrarrojo cercano	758/784	Alexa Fluor™ Plus 750
B7474	Ninguno	Ninguno	Biotin-XX
P3457	Ninguno	Ninguno	Faloidina (sin marcar)

23 opciones

Número de catálogo B3475

Precio (MXN)

Color:

Rojo

Intervalo de longitud de onda de excitación:

558⁄569

Tipo de colorante:

BODIPY™

Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
Ease of use—staining is straightforward and quick
Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo

Tipo de coloranteBODIPY™

Intervalo de longitud de onda de excitación558⁄569

Para utilizar con (equipo)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with Texas Red filter set

Línea de productosBODIPY

Cantidad300 Units

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaColorantes BODIPY

Tipo de productoFaloidina

Sub Cellular LocalizationActina, citoesqueleto, Cytoskeleton

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

Do you offer alternative package sizes for phalloidin or ReadyProbes reagents?

Alternative package sizes may be available through a custom request process. Contact Thermo Fisher Scientific customer support or your sales representative to discuss available options for the specific reagent and quantity needed.

Can I use conjugated phalloidin label probes on organoids?

Yes. Conjugated phalloidin labeling probes can be used with organoids when F-actin is accessible after fixation and permeabilization. Because organoids are thicker than monolayer cell cultures, protocol optimization may be needed, including permeabilization conditions, probe incubation time, and probe concentration.

Are conjugated phalloidin reagents antibodies?

No, Phalloidin is not an antibody. It is a bicyclic peptide toxin originally isolated from the Amanita phalloides mushroom and is used to bind and label F-actin in fixed and permeabilized samples.

Can I use methanol instead of DMSO to reconstitute the conjugated phalloidins from the lyophilized solids?

Yes, methanol can be used to reconstitute fluorescent phalloidin, but it creates a less concentrated stock solution than DMSO. Dissolve the vial contents in 1.5 mL of methanol to yield a 40X stock solution at a concentration of 200 assays/mL, which is equivalent to approximately 6.6 µM. One unit/assay of fluorescent phalloidins is equivalent to 5 µL of the methanolic stock solution. Methanol may be used as a stock-solution solvent, but methanol-containing fixatives are not recommended because they can disrupt F-actin.

How do I reconstitute fluorescent conjugated phalloidin probes to make a stock solution?

For fluorescent phalloidin label probes, prepare a DMSO stock solution by dissolving the vial contents in 150 µL anhydrous DMSO to make a 400X stock, with a concentration of approximately 66 µM or 2,000 assays per mL. Store between -25°C and -5°C, protected from light. One unit/assay of fluorescent phalloidins is equivalent to 0.5 µL of the DMSO stock solution.

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Citations & References (21)

Citations & References

Abstract

Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts.

Authors:Sakaguchi M, Miyazaki M, Inoue Y, Tsuji T, Kouchi H, Tanaka T, Yamada H, Namba M

Journal:J Cell Biol

PubMed ID:10851017

'Many lines of evidence indicate that neoplastic transformation of cells occurs by a multistep process. For neoplastic transformation of normal human cells, they must be first immortalized and then be converted into neoplastic cells. It is well known that the immortalization is a critical step for the neoplastic transformation of ... More

CD34+ corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers.

Authors:Sosnová M, Bradl M, Forrester JV

Journal:Stem Cells

PubMed ID:15790772

'Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, ... More

Real-time gene expression analysis in human xenografts for evaluation of histone deacetylase inhibitors.

Authors:Beliën A, De Schepper S, Floren W, Janssens B, Mariën A, King P, Van Dun J, Andries L, Voeten J, Bijnens L, Janicot M, Arts J

Journal:Mol Cancer Ther

PubMed ID:16985066

'Real-time analysis of gene expression in experimental tumor models represents a major tool to document disease biology and evaluate disease treatment. However, monitoring gene regulation in vivo still is an emerging field, and thus far it has not been linked to long-term tumor growth and disease outcome. In this report, ... More

Suppression of Fas/APO-1-mediated apoptosis by mitogen-activated kinase signaling.

Authors:Holmström TH, Chow SC, Elo I, Coffey ET, Orrenius S, Sistonen L, Eriksson JE

Journal:J Immunol

PubMed ID:9510160

'Jurkat T cells undergo rapid apoptosis upon stimulation of the Fas/APO-1 (CD95) receptor. We examined the role of the mitogen-activated protein kinase (MAPK) cascade as a negative regulator of Fas-mediated apoptosis. To this end, we used both physiologic and artificial activators of MAPK, all of which activate MAPK by distinct ... More

Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell.

Authors:Pucovský V, Harhun MI, Povstyan OV, Gordienko DV, Moss RF, Bolton TB,

Journal:J Cell Mol Med

PubMed ID:17760838

This work aimed to establish the lineage of cells similar to the interstitial cells of Cajal (ICC), the arterial ICC-like (AIL) cells, which have recently been described in resistance arteries, and to study their location in the artery wall. Segments of guinea-pig mesenteric arteries and single AIL cells freshly isolated ... More

View all Phalloidin Labeling Probes citations