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Citas y referencias (173)
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Brefeldina A (de Penicillium brefeldianum)
El metabolito fúngico Brefeldin A (BFA) tiene múltiples objetivos en las células y ha demostrado ser valioso para la disecciónMás información
| Número de catálogo | Cantidad |
|---|---|
| B7450 | 5 mg |
Número de catálogo B7450
Precio (MXN)
-
Cantidad:
5 mg
El metabolito fúngico Brefeldin A (BFA) tiene múltiples objetivos en las células y ha demostrado ser valioso para la disección de los procesos celulares, incluyendo la formación de vesículas y la distribución de quinesinas que participan en la exportación de proteínas recientemente sintetizadas. Nos complace ofrecer el Brefeldin A natural aislado de Penicillium brefeldianum. La exposición de las células al BFA provoca una distorsión en el tráfico de proteínas intracelulares desde el ER al aparato Golgi y la pérdida eventual de la morfología del aparato Golgi; la eliminación del BFA invierte completamente estos efectos. El BFA también altera la morfología de los endosomas y lisosomas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de etiquetaSin marcar
Fórmula molecularC16H24O4
Peso molecular280.36
Cantidad5 mg
Almacenamiento recomendadoAlmacenar en congelador (de -5 °C a -30 °C)
Condiciones de envíoTemperatura ambiente
Forma físicaPolvo
Tipo de productoEsfingolípido
Unit SizeEach
Citations & References (173)
Citations & References
Abstract
Nocodazole-dependent transport, and brefeldin A--sensitive processing and sorting, of newly synthesized membrane proteins in cultured neurons.
Journal:J Neurosci
PubMed ID:7790910
The envelope glycoproteins of Semliki Forest virus (SFV), Vesicular Stomatitis virus (VSV), and Influenza Fowl Plague virus (FPV) are vectorially targeted in neurons to the plasma membrane of dendrites (SFV and VSV) and axons (FPV). To gain insight into the mechanisms responsible for such polarized delivery we have examined the
Mammalian Cdc42 is a brefeldin A-sensitive component of the Golgi apparatus.
Journal:J Biol Chem
PubMed ID:8900167
'In this study, we have used immunocytochemical and fractionation approaches to provide a description of the localization of the mammalian Cdc42 protein (designated Cdc42Hs) in vivo. A specific anti-peptide antibody was generated against the C-terminal region of Cdc42Hs. Using affinity-purified preparations of this antibody in indirect immunofluorescence experiments, Cdc42Hs was
Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome.
Journal:Arthritis Rheum
PubMed ID:9324025
'OBJECTIVE: To identify a Golgi complex autoantigen bound by Sjögren''s syndrome (SS) autoantibodies. METHODS: Serum from a patient with secondary SS and anti-Golgi antibodies was used as a probe to isolate a complementary DNA (cDNA) insert from a HeLa cDNA library. RESULTS: A 3.7-kb cDNA encoding a 56-kd recombinant protein
An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle.
Journal:J Cell Biol
PubMed ID:9585414
'During mitosis, the ribbon of the Golgi apparatus is transformed into dispersed tubulo-vesicular membranes, proposed to facilitate stochastic inheritance of this low copy number organelle at cytokinesis. Here, we have analyzed the mitotic disassembly of the Golgi apparatus in living cells and provide evidence that inheritance is accomplished through an
Direct pathway from early/recycling endosomes to the Golgi apparatus revealed through the study of shiga toxin B-fragment transport.
Journal:J Cell Biol
PubMed ID:9817755
'Shiga toxin and other toxins of this family can escape the endocytic pathway and reach the Golgi apparatus. To synchronize endosome to Golgi transport, Shiga toxin B-fragment was internalized into HeLa cells at low temperatures. Under these conditions, the protein partitioned away from markers destined for the late endocytic pathway