Human Epidermal Keratinocytes, neonatal (HEKn)

Los queratinocitos epidérmicos humanos normales primarios y neonatales (HEKn) se aíslan del prepucio neonatal y se crioconservan al final delMás información

Número de catálogo	Cantidad
C0015C	1 vial

Número de catálogo C0015C

Precio (MXN)

Cantidad:

1 vial

Los queratinocitos epidérmicos humanos normales primarios y neonatales (HEKn) se aíslan del prepucio neonatal y se crioconservan al final del cultivo primario.

Las células:
• Se han comprobado en cuanto a rendimiento: al menos 30 duplicaciones de población cuando se cultiva en el medio EpiLife complementado con HKGS (para HEKn) o suplemento S7 (para HEKn-AOF)
• Se garantiza que sean ≥70 % viables
• Se han comprobado en cuanto a micoplasma, bacterias, levaduras u otros hongos, hepatitis B, hepatitis C y virus VIH-1

Cada vial contiene más de 0,5 x 10⁶ células viables que se han crioconservado al final de la fase de cultivo primario en un medio que contiene DMSO al 10 %.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Fuente de dadoresDonante único

N.° de celdas≥500000

Línea de productosCascade Biologics

Tipo de productoQueratinocito epidérmico humano

Cantidad1 vial

Probado paraHepatitis B (VHB), Hepatitis C (VHC), pruebas de esterilidad (hongos y bacterias), micoplasma, ensayo de rendimiento del crecimiento, VIH-1

AgeRecién nacido (14 días o menos)

Tipo de célulaCélulas de queratinocitos

Culture TypeCultivo celular adherente

FormularioCrioconservado

EspecieHumano

Unit SizeEach

Contenido y almacenamiento

Almacenar en nitrógeno líquido.

Preguntas frecuentes

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.

View all Human Epidermal Keratinocytes, neonatal (HEKn) FAQs

Citations & References (1)

Citations & References

Abstract

High Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter.

Authors:Apt D, Watts RM, Suske G, Bernard HU,

Journal:Virology

PubMed ID:8862423

Gene expression of human papillomavirus type 16 (HPV-16) and other HPV types is epithelial specific. Specificity is brought about by synergism between several different transcription factors that seem to occur ubiquitously but differ qualitatively and quantitatively between cells in which HPV genomes are transcriptionally active or inactive. Here, we report ... More