Human Dermal Fibroblasts, neonatal (HDFn)

Fibroblastos dérmicos humanos primarios aislados de prepucio neonatal crioconservados al final del cultivo primario. • Viabilidad de al menos elMás información

Número de catálogo	Cantidad
C0045C	1 vial

Número de catálogo C0045C

Precio (MXN)

Cantidad:

1 vial

Fibroblastos dérmicos humanos primarios aislados de prepucio neonatal crioconservados al final del cultivo primario.
• Viabilidad de al menos el 70 %
• ≥ 500 000 células viables/vial
• Capaz de al menos 16 duplicaciones de población
• Micoplasma: no se detecta
• Hepatitis B: no se detecta
• Hepatitis C: no se detecta
• VIH-1: no se detecta
• Bacterias, levaduras y otros hongos: no detectados

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Fuente de dadoresDonante único

N.° de celdas≥500000

Línea de productosCascade Biologics

Tipo de productoFibroblastos dérmicos humanos, neonatales

Cantidad1 vial

Probado paraHepatitis B (VHB), Hepatitis C (VHC), pruebas de esterilidad (hongos y bacterias), micoplasma, ensayo de rendimiento del crecimiento, VIH-1

AgeRecién nacido (14 días o menos)

Tipo de célulaCélulas de fibroblastos (dérmica)

Culture TypeCultivo celular adherente

FormularioCrioconservado

EspecieHumano

Unit SizeEach

Contenido y almacenamiento

Temperatura del nitrógeno líquido

Preguntas frecuentes

Can I get passage information for Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C)?

Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C) are primary human dermal fibroblasts isolated from adult skin and neonatal foreskin, respectively. They are cryopreserved at the end of the primary culture stage and not passaged.

What are your Human Dermal Fibroblast (HDF) cells grown in?

HDF cells are grown in Medium 106 with the addition of Low Serum Growth Supplement.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

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