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Citas y referencias (8)
Invitrogen™
OPP (O-propargil-puromicina)
La puromicina o-propargil (OPP) es un alquino análogo de puromicina que se incorpora de forma eficaz a las proteínas reciénMás información
| Número de catálogo | Cantidad |
|---|---|
| C10459 | 5 mg |
Número de catálogo C10459
Precio (MXN)
-
Cantidad:
5 mg
La puromicina o-propargil (OPP) es un alquino análogo de puromicina que se incorpora de forma eficaz a las proteínas recién traducidas en medios completos que contienen metionina. Cuando se utiliza con los ensayos de síntesis de proteínas Click-iT® Plus OPP Alexa Fluor® o los kits de herramientas de azida picolil Alexa Fluor®, la OPP proporciona un método rápido, sensible y no radiactivo para la detección de síntesis de proteínas mediante microscopía de fluorescencia, imágenes de alto contenido o citometría de flujo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Cantidad5 mg
Tipo de reactivoReactivo de etiquetado de proteínas nacientes
Para utilizar con (aplicación)Microscopía de fluorescencia, Adquisición de imágenes de alto contenido, Citometría de flujo
Línea de productosPuromicina o-propargil (OPP)
Tipo de productoOPP
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador entre -5 °C y -30 °C y desecar.
Citations & References (8)
Citations & References
Abstract
Transcriptome analysis of a CHO cell line expressing a recombinant therapeutic protein treated with inducers of protein expression.
Journal:
PubMed ID:26325199
'The search for specific productivity (qP) determinants in Chinese hamster ovary (CHO) cells has been the focus of the biopharmaceutical cell line engineering efforts aimed at creating '
Suppression of the GTPase-activating protein RGS10 increases Rheb-GTP and mTOR signaling in ovarian cancer cells.
Journal:
PubMed ID:26319900
The regulator of G protein signaling 10 (RGS10) protein is a GTPase activating protein that accelerates the hydrolysis of GTP and therefore canonically inactivates G proteins, ultimately terminating signaling. Rheb is a small GTPase protein that shuttles between its GDP- and GTP-bound forms to activate mTOR. Since RGS10 suppression augments
Nucleolar Enrichment of Brain Proteins with Critical Roles in Human Neurodevelopment.
Journal:Mol Cell Proteomics
PubMed ID:27053602
'To study nucleolar involvement in brain development, the nuclear and nucleolar proteomes from the rat cerebral cortex at postnatal day 7 were analyzed using LC-MS/iTRAQ methodology. Data of the analysis are available via ProteomeXchange with identifier PXD002188. Among 504 candidate nucleolar proteins, the overrepresented gene ontology terms included such cellular
The VHL short variant involves in protein quality control.
Journal:Gene
PubMed ID:27196060
'The von Hippel-Lindau (VHL) is the most important and frequently mutated gene in human clear cell renal cell carcinoma (ccRCC). In contrast to its long counterpart, the internal translational variant of VHL protein (VHLs) is evolutionarily conserved. Herein we present evidence that VHLs associates with ribosome complex via interaction with
Requirement of Neuronal Ribosome Synthesis for Growth and Maintenance of the Dendritic Tree.
Journal:J Biol Chem
PubMed ID:26757818
'The nucleolus serves as a principal site of ribosome biogenesis but is also implicated in various non-ribosomal functions, including negative regulation of the pro-apoptotic transcription factor p53. Although disruption of the nucleolus may trigger the p53-dependent neuronal death, neurotoxic consequences of a selective impairment of ribosome production are unclear. Here,