CellROX™ Green Flow Cytometry Assay Kit
CellROX™ Green Flow Cytometry Assay Kit
Citas y referencias (12)
Invitrogen™

CellROX™ Green Flow Cytometry Assay Kit

El kit de ensayos de citometría de flujo CellROX™ Green permite detectar especies reactivas de oxígeno (ROS) en células vivasMás información
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Número de catálogoCantidad
C10492100 ensayos
Número de catálogo C10492
Precio (MXN)
-
Cantidad:
100 ensayos
El kit de ensayos de citometría de flujo CellROX™ Green permite detectar especies reactivas de oxígeno (ROS) en células vivas mediante la citometría de flujo. El kit incluye el nuevo reactivo CellROX™ Green fluorogénico, la tinción de células muertas SYTOX™ Red, N-acetilcisteína (un antioxidante para el control negativo) y la solución de hidroperóxido de butilo terciario (TBHP, un inductor de ROS).

Consulte la guía de selección para todos los reactivos y kits CellROX™.

El kit de ensayos de citometría de flujo CellROX™ Green incluye:

• Sonda fluorogénica formulada para la citometría de flujo que se oxida en presencia de ROS
• Compatibilidad multicolor: apenas existe solapamiento con los fluoróforos que se excitan con otras líneas de láser, lo que facilita el multiplexing con otros reactivos
• Protocolo sencillo: las células se pueden teñir en medios completos o en otros tampones adecuados, por lo que no es necesario utilizar medios sin suero

El reactivo de detección CellROX™ Green es permeable a las células y no fluorescente o muy débilmente fluorescente mientras está en el estado reducido. Tras la oxidación, el reactivo muestra una intensa señal fluorogénica que tiene una absorción / emisión máximas de 508/525 nm y se mantiene en el núcleo y en el citoplasma. Cuando se utiliza junto con la tinción de células muertas SYTOX™ Red, las células con y sin estrés oxidativo se pueden distinguir con fiabilidad de las células muertas con ayuda de un citómetro de flujo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
FormatoTubo
Cantidad100 ensayos
Excitation/EmissionCellROX verde: 508/525, SYTOX rojo: 640/658
Unit SizeEach

Preguntas frecuentes

What are the fluorescence excitation/emission maxima for the CellROX Green Reagent provided in the CellROX Green Flow Cytometry Assay Kit?

The excitation/emission maxima for the CellROX Green Reagent are 508/525 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can CellROX Green dye diluted in DMSO be subjected to freeze-thaw cycles?

CellROX Green dye diluted in a DMSO stock solution is stable for multiple freeze-thaw cycles, but it is not stable long-term in aqueous solution. Please note, every time the stock solution is opened, some of the dye can oxidize, which will increase background noise. DMSO readily absorbs moisture from the air, especially when cold, so be sure to completely thaw the stock solution to room temperature before opening and only open briefly. Water in the DMSO will gradually cause the dye to precipitate and come out of solution. Minimize the number of times you use a stock solution to several freeze-thaw cycles or make small aliquots to reduce the number of freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

What is the difference between the CellROX Green Flow Cytometry Assay Kit (Cat. No. C10492) and CellROX Green Reagent, for oxidative stress detection (Cat. No. C10444)?

The main difference between the CellROX Green Flow Cytometry Assay Kit (Cat. No. C10492) and CellROX Green Reagent, for oxidative stress detection (Cat. No. C10444), is the suggested final working concentrations and application. The CellROX Green in the CellROX Green Flow Cytometry Assay Kit is specifically designed for flow cytometry analysis and is used at a lower concentration than what is required for imaging, as fluorescence-activated cell sorting (FACS) is a very sensitive detection system.

The standalone CellROX Green Reagent (Cat. No. C10444) is marketed for fluorescent microscopy and require working concentrations appropriate for imaging. This can typically be as much as 10-fold higher than the concentration recommended for flowcytometry. The standalone CellROX Green Reagent can also be used for flowcytometry; however, the working concentration will have to be optimized. For the flow cytometry quick reference, use the link below.

CellROX Flow Cytometry Assay Kit Quick Reference

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellROX™ Green Flow Cytometry Assay Kit FAQs

Citations & References (12)

Citations & References
Abstract
Using transcriptomics to improve butanol tolerance in Synechocystis sp. PCC 6803.
Authors:Anfelt J, Hallström B, Nielsen JB, Uhlén M, Hudson EP,
Journal:
PubMed ID:24056459
Cyanobacteria are emerging as promising hosts for production of advanced biofuels such as n-butanol and alkanes. However, cyanobacteria suffer from the same product inhibition problems that plague other microbial biofuel hosts. High concentrations of butanol severely reduce the growth and even low amounts can negatively affect metabolic processes. An understanding ... More
MTI-101 (Cyclized HYD1) Binds a CD44 Containing Complex and Induces Necrotic Cell Death in Multiple Myeloma.
Authors:Gebhard AW, Jain P, Nair RR, Emmons MF, Argilagos RF, Koomen JM, McLaughlin ML, Hazlehurst LA,
Journal:
PubMed ID:24048737
Our laboratory recently reported that treatment with the d-amino acid containing peptide HYD1 induces necrotic cell death in multiple myeloma cell lines. Because of the intriguing biological activity and promising in vivo activity of HYD1, we pursued strategies for increasing the therapeutic efficacy of the linear peptide. These efforts led ... More
Electrochemical Potential Gradient as a Quantitative in Vitro Test Platform for Cellular Oxidative Stress.
Authors:Bryant C, Atha D, Reipa V
Journal:Antioxidants (Basel)
PubMed ID:27409641
Oxidative stress in a biological system is often defined as a redox imbalance within cells or groups of cells within an organism. Reductive-oxidative (redox) imbalances in cellular systems have been implicated in several diseases, such as cancer. To better understand the redox environment within cellular systems, it is important to ... More
Production of Superoxide in Bacteria Is Stress- and Cell State-Dependent: A Gating-Optimized Flow Cytometry Method that Minimizes ROS Measurement Artifacts with Fluorescent Dyes.
Authors:McBee ME, Chionh YH, Sharaf ML, Ho P, Cai MW, Dedon PC
Journal:Front Microbiol
PubMed ID:28377755
The role of reactive oxygen species (ROS) in microbial metabolism and stress response has emerged as a major theme in microbiology and infectious disease. Reactive fluorescent dyes have the potential to advance the study of ROS in the complex intracellular environment, especially for high-content and high-throughput analyses. However, current dye-based ... More
Coral cell separation and isolation by fluorescence-activated cell sorting (FACS).
Authors:Rosental B, Kozhekbaeva Z, Fernhoff N, Tsai JM, Traylor-Knowles N
Journal:BMC Cell Biol
PubMed ID:28851289
Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and ... More
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