Sondas fluorescentes CellTracker™
Sondas fluorescentes CellTracker™
Citas y referencias (31)
Invitrogen™

Sondas fluorescentes CellTracker™

Es un colorante fluorescente adecuado para supervisar el movimiento de las células o su ubicación.
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Número de catálogoCantidadTipo de colorante
C3456520 × 15 μgCellTracker™ Deep Red
C21105 mgCellTracker™ Blue CMAC
C21115 mgCellTracker™ Blue CMHC
C128815 mgCellTracker Blue CMF2HC
C702520 x 50 μgCellTracker Green CMFDA
C29251mgCellTracker Green CMFDA
C21025 mgColorantes BODIPY
C3455120 × 50 μgCellTracker Orange CMRA
C29271 mgCellTracker Orange CMTMR
C3455220 × 50 μgCellTracker™ Red CMTPX
C100945 x 0,1 mgThiolTracker™ Violet
Número de catálogo C34565
Precio (MXN)
12,839.15
Each
Añadir al carro de la compra
Cantidad:
20 × 15 μg
Tipo de colorante:
CellTracker™ Deep Red
Precio (MXN)
12,839.15
Each
Añadir al carro de la compra
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares.
  • Los espectros de excitación/emisión verde (492/517 nm maxima) son ideales para el multiplexing con proteínas y colorantes rojo fluorescente.
  • Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células.
  • Retención de la señal fluorescente superior a >72 horas (normalmente de tres a seis generaciones).
  • Baja citotoxicidad: no afecta a la viabilidad ni a la proliferación
  • El tinte fluorescente de CMAC CellTracker Blue se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impermeabilizantes de membranas celulares.
  • El colorante se transfiere a las células hijas, pero no a las celdas adyacentes de una población.
  • Estable, no tóxico a las concentraciones de trabajo, bien conservado en las células, y brillante fluorescente a pH fisiológico

Adhesión celular, análisis celular, proliferación celular, marcado y seguimiento celular, viabilidad celular y citotoxicidad, viabilidad celular, proliferación y función, Imágenes celulares, ensayos de toxicología celular, quimiotaxis y migración celular, descubrimiento y desarrollo de fármacos, marcado celular general, detección de glutatión, detección de alto contenido (HCS), inmunofluorescencia (IF), tinción y detección de inmunofluorescencia, señalización y homeostomía, señalización, identificación y señalización Seguimiento microbiano, estrés nitrooxidativo, ensayos ADME/Tox basados en dianas, detección de pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorDeep Red
DescripciónCellTracker™ Deep Red Dye, 20 x 15 μg
Método de detecciónFluorescente
Tipo de coloranteCellTracker™ Deep Red
Emisión660 nm
Intervalo de longitud de onda de excitación630 nm
Para utilizar con (aplicación)Imaging
FormularioDry Powder
Línea de productosCellTracker
Cantidad20 × 15 μg
Tipo de reactivoCell Tracker Compounds
Tipo de etiquetaOther Labels or Dyes
Tipo de productoDye
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C, desecar y proteger de la luz.

Preguntas frecuentes

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

Can the CellTracker probes last longer than 72 hours? Can the signal be lost in less than 72 hours?

The retention of signal is not based on time, but rather on the rate of cell division over time and, for some cell types, on the rate of active efflux, protein turnover or other metabolic events. Dependent upon the cell type, it may be possible to see signal that lasts far beyond 72 hours. On the other hand, it may also disappear sooner than 72 hours.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The CellTracker Deep Red Dye signal on my live cells is quite robust, but signal drops after fixation/permeabilization. It is noted that this reagent is well-retained upon fixation, but why I am seeing a loss of some signal?

The CellTracker reagents have a reactive group that will allow covalent binding to various cellular components, large and small. When bound to small cellular components, if these are not cross-linked upon fixation, they may leach out, hence a loss of some signal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Sondas fluorescentes CellTracker™ FAQs

Citations & References (31)

Citations & References
Abstract
Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies.
Authors:Di Buduo CA, Wray LS, Tozzi L, Malara A, Chen Y, Ghezzi CE, Smoot D, Sfara C, Antonelli A, Spedden E, Bruni G, Staii C, De Marco L, Magnani M, Kaplan DL, Balduini A,
Journal:
PubMed ID:25575540
'We present a programmable bioengineered three-dimensional silk based bone marrow niche tissue system that successfully mimics the physiology of human bone marrow environment allowing us to manufacture functional human platelets ex vivo. Using stem/progenitor cells, megakaryocyte function and platelet generation were recorded in response to variations in extracellular matrix components, ... More
Regulation of Phagocytosis in Macrophages by Membrane Ethanolamine Plasmalogens.
Authors:Rubio JM, Astudillo AM, Casas J, Balboa MA, Balsinde J,
Journal:Front Immunol
PubMed ID:30087680
'Macrophages, as professional phagocytes of the immune system, possess the ability to detect and clear invading pathogens and apoptotic cells through phagocytosis. Phagocytosis involves membrane reorganization and remodeling events on the cell surface, which play an essential role in innate immunity and tissue homeostasis and the control of inflammation. In ... More
Nano-scale microfluidics to study 3D chemotaxis at the single cell level.
Authors:Frick C, Dettinger P, Renkawitz J, Jauch A, Berger CT, Recher M, Schroeder T, Mehling M,
Journal:PLoS One
PubMed ID:29879160
'Directed migration of cells relies on their ability to sense directional guidance cues and to interact with pericellular structures in order to transduce contractile cytoskeletal- into mechanical forces. These biomechanical processes depend highly on microenvironmental factors such as exposure to 2D surfaces or 3D matrices. In vivo, the majority of ... More
Tumor-stroma interactions differentially alter drug sensitivity based on the origin of stromal cells.
Authors:Landry BD, Leete T, Richards R, Cruz-Gordillo P, Schwartz HR, Honeywell ME, Ren G, Schwartz AD, Peyton SR, Lee MJ,
Journal:Mol Syst Biol
PubMed ID:30082272
'Due to tumor heterogeneity, most believe that effective treatments should be tailored to the features of an individual tumor or tumor subclass. It is still unclear, however, what information should be considered for optimal disease stratification, and most prior work focuses on tumor genomics. Here, we focus on the tumor ... More
Neutrophil-generated HOCl leads to non-specific thiol oxidation in phagocytized bacteria.
Authors:Degrossoli A, Müller A, Xie K, Schneider JF, Bader V, Winklhofer KF, Meyer AJ, Leichert LI,
Journal:Elife
PubMed ID:29506649
'Phagocytic immune cells kill pathogens in the phagolysosomal compartment with a cocktail of antimicrobial agents. Chief among them are reactive species produced in the so-called oxidative burst. Here, we show that bacteria exposed to a neutrophil-like cell line experience a rapid and massive oxidation of cytosolic thiols. Using roGFP2-based fusion ... More
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