Ensayo de proliferación celular CyQUANT™ NF
Ensayo de proliferación celular CyQUANT™ NF
Citas y referencias (15)
Invitrogen™

Ensayo de proliferación celular CyQUANT™ NF

El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de célulasMás información
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Número de catálogoTipo de célulaCantidad
C35006NF Cell1000 Assays
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Microplacas
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C7026For cells in culture1000 ensayos
C7027Cell Lysis Buffer50 mL
Número de catálogo C35006
Precio (MXN)
12,362.52
Each
Añadir al carro de la compra
Tipo de célula:
NF Cell
Cantidad:
1000 Assays
Precio (MXN)
12,362.52
Each
Añadir al carro de la compra
El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de células en una población y la medición de la proliferación en formato de microplaca.

Características del ensayo de proliferación celular CyQUANT™ NF:

• Más sensible que los ensayos MTT o alamarBlue™
• Rango de detección lineal de 100 a 20.000 células por pocillo (microplaca de 96 pocillos)
• Permite completar los ensayos en una hora

Un método rápido y sencillo para medir la proliferación celular
El ensayo de proliferación celular CyQUANT™ NF no requiere lisis celular, incubaciones largas, radioactividad ni eliminación de tinte de las células. El ensayo CyQUANT™ NF elimina el paso de lisis celular con congelación-descongelación del ensayo de proliferación celular CyQUANT™ original utilizando un tinte fijador de ADN y permeable en célula con un reactivo de permeabilización de la membrana de plasma. El ensayo de proliferación celular CyQUANT™ NF se puede utilizar en cualquiera de los formatos de microplacas de 96 pocillos o 384 pocillos, y está disponible en dos configuraciones: un kit de ensayo 200 (C35007) para pequeños tamaños de muestra y un kit de ensayo 1000 (C35006) para aplicaciones de alto rendimiento.

alamarBlue™ es una marca registrada de TREK Diagnostic Systems.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaNF Cell
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
Excitación/emisión480/520 nm
FormatoPlaca de 384 pocillos, placa de 96 pocillos
Cantidad1000 Assays
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Ensayo de proliferación
Para utilizar con (equipo)Lector de microplacas, HTS Reader
Línea de productosCyQUANT
Tipo de productoEnsayo de proliferación celular
Unit SizeEach
Contenido y almacenamiento
•CyQUANT™ NF dye reagent
•Dye delivery reagent
•5X HBSS buffer (Hank’s balanced salt solution)

It is possible to observe precipitate in HBSS buffer. The precipitate does not have an impact on the use of this kit, nor the results that are gathered from its use.

Preguntas frecuentes

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have spectral data for the CyQUANT NF Cell Proliferation Assay kit?

When CyQUANT NF dye is bound to DNA, the approximate excitation/emission is 480/520 nm. It can be detected using standard GFP or FITC filter settings.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (15)

Citations & References
Abstract
KIF14 messenger RNA expression is independently prognostic for outcome in lung cancer.
Authors:Corson TW,Zhu CQ,Lau SK,Shepherd FA,Tsao MS,Gallie BL
Journal:Clinical cancer research : an official journal of the American Association for Cancer Research
PubMed ID:17545527
Functional symbiosis between endothelium and epithelial cells in glomeruli.
Authors:Hirschberg R, Wang S, Mitu GM,
Journal:Cell Tissue Res
PubMed ID:17999087
'In some capillary beds, pericytes regulate endothelial growth. Capillaries with high filtration capacity, such as those in renal glomeruli, lack pericytes. Glomerular endothelium lies adjacent to visceral epithelial cells (podocytes) that are anchored to and cover the anti-luminal surface of the basement membrane. We have tested the hypothesis that podocytes ... More
Flex-Hets differentially induce apoptosis in cancer over normal cells by directly targeting mitochondria.
Authors:Liu T, Hannafon B, Gill L, Kelly W, Benbrook D
Journal:Mol Cancer Ther
PubMed ID:17575110
'Flex-Het drugs induce apoptosis in multiple types of cancer cells, with little effect on normal cells. This apoptosis occurs through the intrinsic mitochondrial pathway accompanied by generation of reactive oxygen species (ROS). The objective of this study was to determine if direct or indirect targeting of mitochondria is responsible for ... More
Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.
Authors:Hu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,
Journal:Nano Lett
PubMed ID:17887715
'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More
Decrease of endogenous vascular endothelial growth factor may not affect glioma cell proliferation and invasion.
Authors:Hong X, Jiang F, Kalkanis SN, Zhang ZG, Zhang X, Zheng X, Mikkelsen T, Jiang H, Chopp M
Journal:J Exp Ther Oncol
PubMed ID:17552362
'Vascular endothelial growth factor (VEGF) is abundantly produced by glioma cells especially glioblastoma, the most malignant form of astrocytoma. VEGF, a well known angiogenic factor, acts in a paracrine fashion on endothelial cells to develop tumor vasculature. However, recent studies have found that several tumor cells express VEGF receptors, and ... More
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