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Citas y referencias (72)
Invitrogen™
ER-Tracker™ Blue-White DPX, para la adquisición de imágenes de células vivas
El colorante ER-Tracker Blue-White DPX es una sonda fotoestable que es selectiva para el retículo endoplásmico (ER) en células vivas.Más información
| Número de catálogo | Cantidad |
|---|---|
| E12353 | 20 x 50 μL |
Número de catálogo E12353
Precio (MXN)
-
Cantidad:
20 x 50 μL
El colorante ER-Tracker Blue-White DPX es una sonda fotoestable que es selectiva para el retículo endoplásmico (ER) en células vivas. Las células se pueden fijar en aldehído después de la tinción con el colorante, pero se perderá una señal de fluorescencia significativa. Con excitación a ∼374 nm, la sonda sensible al medio ambiente muestra emisiones entre 430 y 640 nm. Un filtro DAPI de paso largo funciona mejor.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorAzul
Concentración1 mM
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosER-Tracker
Cantidad20 x 50 μL
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoTinte
SubCellular LocalizationRetículo endoplásmico
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (72)
Citations & References
Abstract
CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
Journal:J Biol Chem
PubMed ID:17215251
'Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we
Microtubules regulate local Ca2+ spiking in secretory epithelial cells.
Journal:J Biol Chem
PubMed ID:10801885
'The role of the cytoskeleton in regulating Ca(2+) release has been explored in epithelial cells. Trains of local Ca(2+) spikes were elicited in pancreatic acinar cells by infusion of inositol trisphosphate through a whole cell patch pipette, and the Ca(2+)-dependent Cl(-) current spikes were recorded. The spikes were only transiently
Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
Journal:Histochem Cell Biol
PubMed ID:17849139
'Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored
TLR9 signals after translocating from the ER to CpG DNA in the lysosome.
Journal:Nat Immunol
PubMed ID:14716310
'Microbial DNA sequences containing unmethylated CpG dinucleotides activate Toll-like receptor 9 (TLR9). We have found that TLR9 is localized to the endoplasmic reticulum (ER) of dendritic cells (DCs) and macrophages. Because there is no precedent for immune receptor signaling in the ER, we investigated how TLR9 is activated. We show
Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
Journal:J Microsc
PubMed ID:12423261
'Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle