EnzChek™ Paraoxonase Assay Kit

El kit de ensayo de paraoxonasa EnzChek™ es un ensayo fluorométrico homogéneo y altamente sensible para la actividad organofosfatasa deMás información

Número de catálogo	Cantidad
E33702	1 kit

Número de catálogo E33702

Precio (MXN)

Cantidad:

1 kit

El kit de ensayo de paraoxonasa EnzChek™ es un ensayo fluorométrico homogéneo y altamente sensible para la actividad organofosfatasa de la paraoxonasa. Basado en la hidrólisis del análogo fluorogénico, este ensayo es > 10 veces más sensible que el ensayo colorimétrico y, a diferencia de este, puede distinguir muestras de actividad de la paraoxonasa muy similares. En condiciones estándar, el ensayo requiere solo 5 µl de suero, genera una señal en tan solo 15 minutos y es lineal hasta 50 minutos. El ensayo solo requiere una única reacción, que puede supervisarse continuamente o terminarse utilizando el reactivo de parada incluido en el kit.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónIntensidad de la fluorescencia

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

Tipo de sustratoSustrato de paraoxonasa

Enzima dianaParaoxonasas

Para utilizar con (aplicación)Ensayo de paraoxonasa

Línea de productosEnzChek

Tipo de productoEnsayo de paraoxonasa

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador entre -5 °C y -30 °C

Citations & References (4)

Citations & References

Abstract

Increased serum advanced glycation end products are associated with impairment in HDL antioxidative capacity in diabetic nephropathy.

Authors:Zhou H, Tan KC, Shiu SW, Wong Y,

Journal:Nephrol Dial Transplant

PubMed ID:18065800

'Advanced glycation end products (AGEs) play an important role in the pathogenesis of diabetic complications. Recent data suggest that AGEs may also interfere with the function of HDL and the reverse cholesterol transport pathway. We have investigated whether serum AGE level is associated with impairment in the antioxidative capacity of ... More

An apolipoprotein A-I mimetic dose-dependently increases the formation of prebeta1 HDL in human plasma.

Authors:Troutt JS, Alborn WE, Mosior MK, Dai J, Murphy AT, Beyer TP, Zhang Y, Cao G, Konrad RJ,

Journal:J Lipid Res

PubMed ID:18056684

'Prebeta1 HDL is the initial plasma acceptor of cell-derived cholesterol in reverse cholesterol transport. Recently, small amphipathic peptides composed of D-amino acids have been shown to mimic apolipoprotein A-I (apoA-I) as a precursor for HDL formation. ApoA-I mimetic peptides have been proposed to stimulate the formation of prebeta1 HDL and ... More

Exercise and diet induced weight loss improves measures of oxidative stress and insulin sensitivity in adults with characteristics of the metabolic syndrome.

Authors:Rector RS, Warner SO, Liu Y, Hinton PS, Sun GY, Cox RH, Stump CS, Laughlin MH, Dellsperger KC, Thomas TR,

Journal:Am J Physiol Endocrinol Metab

PubMed ID:17473052

'Obesity and insulin resistance (IR) increase the risk for coronary heart disease; however, much of this risk is not attributable to traditional risk factors. We sought to determine whether weight loss associated with supervised aerobic exercise beneficially alters biomarkers of oxidative stress and whether these alterations are associated with improvements ... More

The antiatherogenic function of HDL is impaired in hyperhomocysteinemic subjects.

Authors:Holven KB, Aukrust P, Retterstøl K, Otterdal K, Bjerkeli V, Ose L, Nenseter MS, Halvorsen B,

Journal:J Nutr

PubMed ID:18936200

High plasma homocysteine concentrations have been associated with increased risk of cardiovascular disease, whereas plasma HDL concentration is inversely correlated to such disorders. We hypothesized that hyperhomocysteinemic subjects may have dysfunctional HDL. We therefore investigated the ability of serum from hyperhomocysteinemic male and female subjects (n = 10) and control ... More