ER-Tracker™ Red (BODIPY™ TR Glibenclamide), para la adquisición de imágenes de células vivas

Citas y referencias (28)

Invitrogen™

ER-Tracker™ Red (BODIPY™ TR Glibenclamide), para la adquisición de imágenes de células vivas

El colorante rojo ER-Tracker™ es una tinción de células vivas que penetra en la célula y es altamente selectiva paraMás información

Número de catálogo	Cantidad
E34250	100 μg

Número de catálogo E34250

Precio (MXN)

Cantidad:

100 μg

El colorante rojo ER-Tracker™ es una tinción de células vivas que penetra en la célula y es altamente selectiva para el retículo endoplásmico (RE) que, cuando se tiñe según el protocolo proporcionado, conserva parcialmente el patrón de tinción después de la fijación con formaldehído. Este tinte consta del colorante verde fluorescente BODIPY™ TR y de glibenclamida. La glibenclamida (gliburida) se une a los receptores de sulfonilurea de canales K⁺ sensibles al adenosín trifosfato (ATP) que son prominentes en ER; la actividad farmacológica de la glibenclamida podría afectar potencialmente la función de ER. La expresión variable de receptores de sulfonilurea en algunos tipos de células especializadas puede resultar en un etiquetado sin ER.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo

Método de detecciónFluorescente

Intervalo de longitud de onda de excitación587⁄615

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosBODIPY, ER-Tracker

Cantidad100 μg

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaBODIPY Dyes

Tipo de productoTinte

SubCellular LocalizationRetículo endoplásmico

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (28)

Citations & References

Abstract

Binding of guanylyl cyclase activating protein 1 (GCAP1) to retinal guanylyl cyclase (RetGC1). The role of individual EF-hands.

Authors:Peshenko IV, Olshevskaya EV, Dizhoor AM,

Journal:J Biol Chem

PubMed ID:18541533

'Guanylyl cyclase activating protein 1 (GCAP1), after substitution of Ca(2+) by Mg(2+) in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. We inactivated metal binding in individual EF-hands of GCAP1 tagged with green fluorescent protein to assess their role in GCAP1 binding to RetGC1 in co-transfected HEK293 ... More

Imaging secretory vesicles by fluorescent protein insertion in propeptide rather than mature secreted peptide.

Authors:Watkins S, Geng X, Li L, Papworth G, Robbins PD, Drain P

Journal:Traffic

PubMed ID:12047554

'We combined confocal and live-cell imaging with a novel molecular strategy aimed at revealing mechanisms underlying glucose-regulated insulin vesicle secretion. The ''Ins-C-GFP'' reporter monitors secretory peptide targeting, trafficking, and exocytosis without directly tagging the mature secreted peptide. We trapped a green fluorescent protein (GFP) reporter in equimolar quantity within the ... More

Faecal excretion of ciprofloxacin after a single oral dose and its effect on faecal bacteria in healthy volunteers.

Authors:Pecquet S, Ravoire S, Andremont A,

Journal:J Antimicrob Chemother

PubMed ID:2211433

'High concentrations of ciprofloxacin have been shown to persist in the faeces of volunteers for several days after a week of oral treatment with this drug, which was also found to have a prolonged effect on aerobic Gram-negative intestinal bacteria. To determine whether a shorter course of ciprofloxacin would have ... More

Insulin-like growth factor I enhances the expression of aromatase P450 by inhibiting autophagy.

Authors:Zhang B, Shozu M, Okada M, Ishikawa H, Kasai T, Murakami K, Nomura K, Harada N, Inoue M,

Journal:Endocrinology

PubMed ID:20668023

'Aromatase, a key enzyme of estrogen biosynthesis, is transcriptionally regulated by many growth factors. IGF-I enhances aromatase activity in a variety of cells, but the mechanism of action has not been determined. We herein report our finding of a novel mechanism of action for IGF-I. IGF-I enhanced the dexamethasone (DEX)-induced ... More

Essential requirement for sphingosine kinase 2 in a sphingolipid apoptosis pathway activated by FTY720 analogues.

Authors:Don AS, Martinez-Lamenca C, Webb WR, Proia RL, Roberts E, Rosen H

Journal:J Biol Chem

PubMed ID:17400555

'The clinical immunosuppressant FTY720 is a sphingosine analogue that, once phosphorylated by sphingosine kinase 2 (Sphk2), is an agonist of multiple receptor subtypes for sphingosine 1-phosphate. Short exposures to FTY720 afford long term protection in lymphoproliferative and autoimmune disease models, presumably by inducing apoptosis in subsets of cells essential for ... More

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