Sustrato de fosfatasa ELF™ 97 (fosfato ELF™ 97), filtrado de 0,2 μm
Sustrato de fosfatasa ELF™ 97 (fosfato ELF™ 97), filtrado de 0,2 μm
Citas y referencias (38)
Invitrogen™

Sustrato de fosfatasa ELF™ 97 (fosfato ELF™ 97), filtrado de 0,2 μm

El sustrato de la fosfatasa ELF™ 97 en la hidrólisis produce un precipitado fluorescente amarillo-verde brillante y fotoestable en elMás información
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Número de catálogoCantidad
E65881 ml
Número de catálogo E6588
Precio (MXN)
-
Cantidad:
1 ml
El sustrato de la fosfatasa ELF™ 97 en la hidrólisis produce un precipitado fluorescente amarillo-verde brillante y fotoestable en el sitio de la actividad enzimática. Este precipitado fluorescente tiene varias características espectrales únicas, incluyendo un desplazamiento Stokes extremadamente grande que lo hace fácilmente distinguible de la fluorescencia endógena.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularPenetra en la célula
ColorAmarillo-verde
Concentración5 mM
Excitación/emisión345⁄530
Para utilizar con (equipo)Microscopio de fluorescencia, micromatriz, citómetro de flujo
Etiqueta o tinteELF 97
Línea de productosELF
Cantidad1 ml
Categoría de investigaciónDiferenciación
Condiciones de envíoTemperatura ambiente
SustratoSustrato de fosfatasa
Método de detecciónFluorescente
FormularioMediciones
Substrate PropertiesSustrato químico
Target EnzymeFosfatasas
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (38)

Citations & References
Abstract
Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.
Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM
Journal:Stem cells (Dayton, Ohio)
PubMed ID:16293579
Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
Journal:Stem Cells
PubMed ID:17284651
'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More
Quantitative differences in phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes.
Authors:Ekins S, Murray GI, Burke MD, Williams JA, Marchant NC, Hawksworth GM
Journal:Drug Metab Dispos
PubMed ID:8591730
'Testosterone (250 microM), 7-ethoxycoumarin (25 microM), and 1-chloro-2,4-dinitrobenzene (CDNB, 50 microM) were used as substrates to compare phase I and II metabolism in rat precision-cut liver slices and rat hepatocytes. Overall clearance to metabolites was significantly greater in hepatocytes for testosterone (1.9- to 16.9-fold), 7-ethoxycoumarin (O-deethylation, 14.8-fold; glucuronidation, 3.1-fold), and ... More
Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1.
Authors:Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ
Journal:Clin Chim Acta
PubMed ID:15748605
'OBJECTIVE: As alkaline phosphatase may play a role in cell differentiation, our aim was to study the possible role of this enzyme in the differentiation of preadipocytes (3T3-L1 cells) into adipocytes. RESEARCH METHODS AND PROCEDURES: 3T3-L1 cells were grown in medium containing insulin, dexamethasone and IBMX to induce adipogenesis. Adipogenesis ... More
Fluorescence in situ hybridization of scarce leptin receptor mRNA using the enzyme-labeled fluorescent substrate method and tyramide signal amplification.
Authors:Breininger JF, Baskin DG
Journal:J Histochem Cytochem
PubMed ID:11101627
'To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification (TSA) and Enzyme-Labeled Fluorescent alkaline phosphatase substrate (ELF) methods. First, ... More
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