EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays
Citas y referencias (9)
Invitrogen™

EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

El kit de ensayo de proteasa EnzChek™, de fluorescencia roja, es un ensayo rápido, simple y directo basado en fluorescenciaMás información
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Número de catálogoCantidad
E66391 kit
Número de catálogo E6639
Precio (MXN)
-
Cantidad:
1 kit
El kit de ensayo de proteasa EnzChek™, de fluorescencia roja, es un ensayo rápido, simple y directo basado en fluorescencia para detectar metaloproteasas de serina, ácido y sulfhidrilo. El aumento de fluorescencia que lo acompaña, que puede medirse con un lector de microplacas de fluorescencia, es proporcional a la actividad de la proteasa.

Consulte nuestra línea completa de ensayos de microplacas de fluorescencia.

• El mecanismo generalizado permite la medición de una variedad de proteasas
• Se puede utilizar para medir continuamente la cinética de la proteasa
• Excitación/emisión similar al colorante Texas Red™ (589/617 nm)

Este kit de ensayo de proteasas EnzChek™ contiene un derivado de caseína que se ha etiquetado ampliamente con el colorante rojo fluorescente BODIPY™ TR-X, lo que provoca la desactivación del colorante fluorescente. La hidrólisis catalizada por proteasas libera los péptidos etiquetados con colorante BODIPY™ TR-X muy fluorescente, lo que permite la detección cuantitativa de la actividad de la proteasa en la solución. El colorante rojo fluorescente BODIPY™ TR-X tiene espectros de excitación y emisión similares a los del fluoróforo Texas Red™.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónIntensidad de la fluorescencia
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
Propiedades de sustratoSustrato basado en proteínas
Tipo de sustratoSustrato de proteasa
Enzima dianaProteasas
Para utilizar con (aplicación)Ensayo de proteasa
Para utilizar con (equipo)Lector de microplacas de fluorescencia
Línea de productosEnzChek
Tipo de productoEnsayo de proteasa
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long is the reconstituted BODIPY substrate in the EnzChek Protease Assay Kit, red fluorescence, 100-1000 assays (Cat. No. E6639) stable?

Reconstituted BODIPY casein substrates may be stored at 4 degrees C for 2-4 weeks. We recommend the addition of sodium azide at a final concentration of 2 mM to act as a preservative. If longer storage is required, freeze at -20 degrees C. Protect from light and avoid repeated freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (9)

Citations & References
Abstract
Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.
Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP
Journal:Anal Biochem
PubMed ID:9299009
'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More
Subnanoliter enzymatic assays on microarrays.
Authors:Angenendt P, Lehrach H, Kreutzberger J, Glökler J
Journal:Proteomics
PubMed ID:15700241
'Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation of these assays. However, the final transfer of enzymatic assays ... More
Continuous assay of proteases using a microtiter plate fluorescence reader.
Authors:Menges DA, Ternullo DL, Tan-Wilson AL, Gal S
Journal:Anal Biochem
PubMed ID:9398357
A protease-dependent mechanism for initiating T-dependent B cell responses to large particulate antigens.
Authors:Catron DM, Pape KA, Fife BT, van Rooijen N, Jenkins MK,
Journal:J Immunol
PubMed ID:20208013
Ab production is critical for antimicrobial immunity, and the initial step in this process is the binding of Ag to the BCR. It has been shown that small soluble proteins can directly access the lymph node follicles to reach naive B cells, but virus particles must be translocated into follicles ... More
Selective modulation of superantigen-induced responses by streptococcal cysteine protease.
Authors:Kansal RG, Nizet V, Jeng A, Chuang WJ, Kotb M
Journal:J Infect Dis
PubMed ID:12552423
Streptococcal pyrogenic exotoxin (Spe) B, a streptococcal cysteine protease, is believed to be important in group A streptococcal (GAS) pathogenesis. The present study examined the effect of SpeB on the activity of superantigenic exotoxins secreted by M1T1 GAS isolates. The proliferative response of human lymphocytes to culture supernatant (SUP) from ... More
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