NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well
NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well
Invitrogen™

NuPAGE™ Tris-Acetate Mini Protein Gels, 3 to 8%, 1.0 mm, 2D-well

Los geles de Tris-acetato NuPAGE™ Novex™ al 3–8 % proporcionan una excelente separación de proteínas de peso molecular grande cuandoMás información
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EA0376BOXUnidad
Número de catálogo EA0376BOX
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Los geles de Tris-acetato NuPAGE™ Novex™ al 3–8 % proporcionan una excelente separación de proteínas de peso molecular grande cuando se usan con el tampón de desplazamiento de Tris-acetato NuPAGE™. Los geles de Tris-acetato NuPAGE™ Novex™ también se pueden procesar con el tampón de desplazamiento nativo de Tris-glicina para resolver proteínas nativas de forma más eficaz que con el sistema de gel de Tris-glicina.

Formulación
Los geles de Tris-acetato NuPAGE™ Novex™ se fabrican con reactivos de alta pureza y se someten a estrictos controles de calidad: base de Tris, ácido acético, acrilamida, bisacrilamida, TEMED, SAF y agua altamente purificada. No contienen SDS.

Tampones recomendados
Ejecute los geles de Tris-Acetato NuPAGE™ Novex™ con el tampón de desplazamiento de Tris-acetato NuPAGE™ SDS. Para garantizar una buena reducción de muestras y resolución de banda, utilice con estos geles reactivos para preparación de muestras NuPAGE™. El uso del tampón de transferencia NuPAGE™ proporciona las condiciones óptimas para la transferencia de proteínas a membranas de nailon, nitrocelulosa o PVDF para su posterior análisis. Para las condiciones nativas de procesamiento, los tampones de muestras y de desplazamiento nativos Tris-glicina se deben usar con los geles Tris-acetato NuPAGE™ Novex™.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Gel Thickness1,0 mm
Longitud (métrico)8 cm
Longitud del gel (métrico)8 cm
Modo de separaciónPeso molecular
Línea de productosNovex, NuPAGE
Tipo de productoGradiente Page
CantidadUnidad
Duración de almacenamiento6 meses
Condiciones de envíoHielo húmedo
Tipo de sistemaZOOM™
Grosor1,0 mm
Diseño del pocilloPocillo 2D
Anchura (métrico)8 cm
Anchura del gel (métrico)8 cm
Para utilizar con (equipo)Depósito de minigel, XCell SureLock Mini
Porcentaje del gelDel 3 al 8%
Tamaño de gelMini
Tipo de gelTris-acetato
Intervalo de separaciónDe 36 a 500 kDa
Tipo de separaciónDesnaturalización, nativa
Separación deproteína
PocillosPocillo 2D
Unit SizeEach
Contenido y almacenamiento
Una caja contiene 10 geles. Almacenar en el refrigerador (2–8° C). No la congele. La vida útil es de 6 meses.

Preguntas frecuentes

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What does it mean when bands appear to be getting narrower (or "funneling") as they progress down a protein gel?

There may be too much beta-mercaptoethanol (BME), sample buffer salts, or dithiothreitol (DTT) in your samples. If the proteins are over-reduced, they can be negatively charged and actually repel each other across the lanes causing the bands to get narrower as they progress down the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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