Kit de ensayo de calcio Fluo-4 Direct™
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Citas y referencias (8)
Invitrogen™

Kit de ensayo de calcio Fluo-4 Direct™

El kit de ensayo de calcio Fluo-4 Direct™ se ha formulado para proporcionar un ensayo de calcio fluorescente y homogéneoMás información
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Número de catálogoCantidad
F10472100 ml
F10471100 ml
F104731000 ml
Número de catálogo F10472
Precio (MXN)
-
Cantidad:
100 ml
El kit de ensayo de calcio Fluo-4 Direct™ se ha formulado para proporcionar un ensayo de calcio fluorescente y homogéneo que cumple lo siguiente:
1. Se carga fácilmente en las células
2. Logra una gran ventana de ensayo y
3. Suprime la fluorescencia de fondo generada desde el indicador de calcio en medios completos con poca o ninguna repercusión sobre la fluorescencia celular específica

Esta fórmula avanzada permite que el ensayo se ejecute en un simple formato de «solo adición» en presencia de medios con suero. Fluo-4 Direct™ es la tercera incorporación a la familia de reactivos de detección de calcio Fluo-4. Tanto Fluo-4 AM como Fluo-4 NW requieren la extracción del medio antes la detección del ensayo, pero Fluo-4 NW añade comodidad mediante la inclusión del reactivo PowerLoad™ para facilitar la carga de la célula. El kit de ensayo de calcio Fluo-4 Direct™ es similar a Fluo-4 NW en que ambos están formulados con PowerLoad™ para facilitar la carga de células, pero es único porque puede utilizarse en presencia de los medios de cultivo completos y suprime con eficacia la fluorescencia de fondo sin sacrificar la fluorescencia celular específica generada en el ensayo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteA base de colorantes fluorescentes
FormularioPolvo
ModificaciónProductos químicos
Cantidad100 ml
Condiciones de envíoTemperatura ambiente
Localización subcelularNúcleo, orgánulos, citoplasma
ColorVerde
EmissionVisible
Para utilizar con (aplicación)Ensayo de calcio
Para utilizar con (equipo)Microscopio confocal, Microscopio de fluorescencia, Instrumentos de alto contenido, Lector HTS, Lector de microplacas, Generador de imágenes de fluorescencia
Línea de productosMolecular Probes
Tipo de productoKit de ensayo de calcio
Unit SizeEach
Contenido y almacenamiento
  • Reactivo de ensayo de calcio Fluo-4 Direct™ de 100 ml (componente A)
  • Probenecida de 2 × 77 mg (componente B)
  • Tampón de ensayo de calcio Fluo-4 Direct™ de 200 ml (componente A)
  • Almacenar a ≤ -20 °C. Desecar y proteger de la luz.

Preguntas frecuentes

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (8)

Citations & References
Abstract
High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.
Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,
Journal:J R Soc Interface
PubMed ID:21490003
'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More
Transient receptor potential vanilloid-1 (TRPV1) is a mediator of lung toxicity for coal fly ash particulate material.
Authors:Deering-Rice CE, Johansen ME, Roberts JK, Thomas KC, Romero EG, Lee J, Yost GS, Veranth JM, Reilly CA,
Journal:Mol Pharmacol
PubMed ID:22155782
'Environmental particulate matter (PM) pollutants adversely affect human health, but the molecular basis is poorly understood. The ion channel transient receptor potential vanilloid-1 (TRPV1) has been implicated as a sensor for environmental PM and a mediator of adverse events in the respiratory tract. The objectives of this study were to ... More
20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.
Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,
Journal:J Biol Chem
PubMed ID:22389490
'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More
Screening ß-arrestin recruitment for the identification of natural ligands for orphan G-protein-coupled receptors.
Authors:Southern C, Cook JM, Neetoo-Isseljee Z, Taylor DL, Kettleborough CA, Merritt A, Bassoni DL, Raab WJ, Quinn E, Wehrman TS, Davenport AP, Brown AJ, Green A, Wigglesworth MJ, Rees S,
Journal:J Biomol Screen
PubMed ID:23396314
A variety of G-protein-coupled receptor (GPCR) screening technologies have successfully partnered a number of GPCRs with their cognate ligands. GPCR-mediated ß-arrestin recruitment is now recognized as a distinct intracellular signaling pathway, and ligand-receptor interactions may show a bias toward ß-arrestin over classical GPCR signaling pathways. We hypothesized that the failure ... More
A novel in vitro model system for smooth muscle differentiation from human embryonic stem cell-derived mesenchymal cells.
Authors:Guo X, Stice SL, Boyd NL, Chen SY,
Journal:Am J Physiol Cell Physiol
PubMed ID:23220114
The objective of this study was to develop a novel in vitro model for smooth muscle cell (SMC) differentiation from human embryonic stem cell-derived mesenchymal cells (hES-MCs). We found that hES-MCs were differentiated to SMCs by transforming growth factor-ß (TGF-ß) in a dose- and time-dependent manner as demonstrated by the ... More
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