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Citas y referencias (38)
Invitrogen™
Ácido de fluoresceína-5-(y-6)-sulfónico, sal de trisodio
El ácido sulfónico de fluoresceína-5-(y-6) impermeable a células se puede usar como un trazador polar no fijable para investigar laMás información
| Número de catálogo | Cantidad |
|---|---|
| F1130 | 100 mg |
Número de catálogo F1130
Precio (MXN)
-
Cantidad:
100 mg
El ácido sulfónico de fluoresceína-5-(y-6) impermeable a células se puede usar como un trazador polar no fijable para investigar la fusión, la lisis y la comunicación de la brecha-unión.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Fórmula molecularC20H9Na3O8S
Cantidad100 mg
Almacenamiento recomendadoAlmacenar a temperatura ambiente y proteger de la luz.
Condiciones de envíoTemperatura ambiente
Localización subcelularMembranas celulares y lípidos
ColorVerde
Forma físicaSólido
Tipo de productoSal trisódica
Unit SizeEach
Citations & References (38)
Citations & References
Abstract
Rapid development of vasopressin-induced hydroosmosis in kidney collecting tubules measured by a new fluorescence technique.
Journal:Biophys J
PubMed ID:3224145
'The pre-steady-state kinetics of the vasopressin-induced increase in collecting tubule osmotic water permeability (Pf) has been measured by a new fluorescence technique. Isolated cortical collecting tubules (CCT) from rabbit kidney were perfused with physiological buffers containing the impermeant fluorophores fluorescein sulfonate (FS) and pyrenetetrasulfonic acid (PTSA). Tubules were subject to
The delta-isoform of protein kinase C causes inducible nitric-oxide synthase and nitric oxide up-regulation: key mechanism for oxidant-induced carbonylation, nitration, and disassembly of the microtubule cytoskeleton and hyperpermeability of barrier of intestinal epithelia.
Journal:J Pharmacol Exp Ther
PubMed ID:12606598
'Using intestinal (Caco-2) cells, we found that oxidant-induced disruption of barrier integrity requires microtubule disassembly. Protein kinase C (PKC)-delta isoform seems to be essential for disruption, but the mechanism is unknown. Because inducible nitric-oxide synthase (iNOS) is key to oxidant stress, we hypothesized that PKC-delta activation is essential in oxidant-induced
Phospholipase C-gamma inhibition prevents EGF protection of intestinal cytoskeleton and barrier against oxidants.
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:11447022
'Loss of intestinal barrier integrity is associated with oxidative inflammatory GI disorders including inflammatory bowel disease. Using monolayers of human intestinal epithelial (Caco-2) cells, we recently reported that epidermal growth factor (EGF) protects barrier integrity against oxidants by stabilizing the microtubule cytoskeleton, but the mechanism downstream of the EGF receptor
Activation of delta-isoform of protein kinase C is required for oxidant-induced disruption of both the microtubule cytoskeleton and permeability barrier of intestinal epithelia.
Journal:J Pharmacol Exp Ther
PubMed ID:12235228
'Using monolayers of intestinal (Caco-2) cells, we showed that oxidants disassemble the microtubule cytoskeleton and disrupt barrier integrity (permeability) (Banan et al., 2000a). Because exposure of our parental cells to oxidants causes protein kinase C (PKC)-delta to be translocated to particulate fractions, we hypothesized that PKC-delta activation is required for
Transepithelial water permeability in microperfused distal airways. Evidence for channel-mediated water transport.
Journal:J Clin Invest
PubMed ID:8609221
'Water movement across the airway epithelium is important for regulation of the volume and composition of airspace fluid. A novel approach is reported here to measure osmotic and diffusional water permeability in intact airways. Small airways (100-200 microns diameter, 1-2 mm length) from guinea pig lung were microdissected and perfused