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Citas y referencias (144)
Invitrogen™
Fluorescein Diacetate (FDA)
El diacetato de fluoresceína (FDA) es un sustrato de esterasa perdestinada a células que puede servir como viabilidad sonda queMás información
| Número de catálogo | Cantidad |
|---|---|
| F1303 | 1 g |
Número de catálogo F1303
Precio (MXN)
-
Cantidad:
1 g
El diacetato de fluoresceína (FDA) es un sustrato de esterasa perdestinada a células que puede servir como viabilidad sonda que mide tanto la actividad enzimática, que es necesaria para activar su fluorescencia, como la integridad célula-membrana, que es necesaria para la retención intracelular de su producto fluorescente. Tras la hidrólisis por esterasas intracelulares, el éster AM produce fluoresceína
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de coloranteColorantes clásicos
Cantidad1 g
Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular
Condiciones de envíoTemperatura ambiente
Tipo de productoDiacetato de fluoresceína
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador entre -5 °C y -30 °C
Citations & References (144)
Citations & References
Abstract
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Ligand/receptor internalization: a spectroscopic analysis and a comparison of ligand binding, cellular response, and internalization by human neutrophils.
Journal:J Cell Biochem
PubMed ID:6302116
'We have compared the kinetics of the responses of neutrophils to the kinetics of ligand-receptor interaction and internalization, using as a model ligand the fluoresceinated hexapeptide N-CHO-Nle-Leu-Phe-Nle-Tyr-Lys-Fluorescein (Nle, norleucine). Cellular responses, ie, membrane depolarization, enzyme (elastase) secretion, and superoxide anion (O-2) generation, are all initiated within 10 sec of the
Interaction of Mycobacterium avium-containing phagosomes with the antigen presentation pathway.
Journal:J Immunol
PubMed ID:11086039
'Pathogenic mycobacteria infect macrophages where they replicate in phagosomes that minimize contact with late endosomal/lysosomal compartments. Loading of Ags to MHC class II molecules occurs in specialized compartments with late endosomal characteristics. This points to a sequestration of mycobacteria-containing phagosomes from the sites where Ags meet MHC class II molecules.
In vitro testing of chemotherapeutic drug combinations in acute myelocytic leukaemia using the fluorometric microculture cytotoxicity assay (FMCA).
Journal:Br J Cancer
PubMed ID:8494730
'The fluorometric microculture cytotoxicity assay (FMCA) was employed for analysing the effect of different chemotherapeutic drug combinations and their single constituents in 44 cases of acute myelocytic leukaemia (AML). A large heterogeneity with respect to cell kill was observed for all combinations tested, the interactions ranging from antagonistic to synergistic
Fluorimetric quantification of cell death in monolayer cultures and cell suspensions.
Journal:J Biochem Biophys Methods
PubMed ID:1779095
'A fluorimetric assay using ethidium bromide (EB) was employed to quantify cell death in monolayer cell cultures (MA-104 cells) in situ and isolated cell suspensions (isolated colonic cells and Leishmania). Fluorescence of EB stained cells was measured with a photometer coupled to an inverted microscope for cell monolayers or in